Curriculum Vitae
Name: AJAY VIR SINGH
Designation: Scientist ‘C’/ Sr. Research Officer
Discipline: Microbiology and Molecular Biology
Date of Joining April 26, 2013
Date of present post April 26, 2013
Date of Birth: July 1
st
, 1982
Address Office: Department of Microbiology and Molecular Biology, National JALMA
Institute for Leprosy & Other Mycobacterial Diseases (ICMR), Dr. M.
Myazaki Marg, Tajganj , Agra -282004 (INDIA).
Contact Details: Phone: +91 562 2333595, 2331756 (Office)
Fax: +91 562 2331756
Areas of interest: Mycobacterial infections, Molecular epidemiology, Genomics, Diagnostics
development and Host- pathogen interaction.
Qualifications: M.Sc., Ph.D., PDF
Awards, Honors and Fellowships:
1. ‘Young Researcher Award’ by Millennium Indian Education Foundation, New Delhi on
August 12,2016.
2. ‘Post Doctoral Fellowship’ awarded by Indian Council of Medical Research, New Delhi
for the period of August 4, 2010- April 25, 2013.
3. ‘Helping Hand Award, 2011” awarded by International Association for Paratuberculosis
(IAP) for attending the 11th International Colloquium on Paratuberculosis, Sydney,
Australia.
4. ‘Best Oral Presentation Award” in 6th annual conference of association of medical
microbiologist-up chapter in MICROCON-2010 at National JALMA Institute for Leprosy
and Other Mycobacterial Diseases (ICMR), Agra, March 13-14, 2010.
5. ‘Best Poster Presentation Award’ at National Seminar on Rural India Development
Alternatives: Sectoral Convergence for Livelihood Security, at Central Institute for
Research on Goats, Makhdoom, Mathura, January 16-18, 2009.
6. ‘Best Poster presentation Award’ in National seminar on Emerging diseases of Small
ruminant and there containment under WTO Regime, at Central Institute for Research on
Goats, Makhdoom, Mathura, February 3-5,2007.
7. Annual ISSGPU Award for the ‘Best Research Article’ published in the Indian Journal of
Small Ruminants during the year of 2010 for the paper entitled “Evaluation of ‘Blood
PCR’ with Blood culture, ELISA and Microscopic Examination for the diagnosis
of Mycobacterium avium subspecies paratuberculosis infection in goat kids”.
8. “Helping Hand Award, 2007” awarded by International Association for Paratuberculosis
(IAP) for attending the 9th International Colloquium on Paratuberculosis, Tsukuba, Japan.
9. Two years Honorary membership (2007-2008) awarded by International Association for
Paratuberculosis, USA.
Office bearer of scientific committees/ societies and other bodies
1. Member, 'Expert group on 'Crohn's disease vs Johne's disease' Indian Council of Medical
Research, New Delhi.
2. Member, Research Advisory Committee for the program of Model Rural Health Research
Unit (MRHRU), Haroli, Una, Himachal Pradesh.
3. Core committee member, National Working Group on Non-Tuberculous Mycobacteria
(NTM)
4. Member, “Board of Studies” Mangalytayan, University, Aligarh.
5. Expert Member, Research Degree Committee (Life Sciences), Jaipur National University,
Jaipur.
Research projects directed:
1. Proteomic profiling of exosomes for the identification of potential biomarkers for
tuberculosis in HIV patients. (Principal Investigator)
2. Molecular strain characterization and its correlation with drug resistance in tuberculous
and non-tuberculous mycobacteria in HIV positive and HIV negative patients in Agra
region. (Principal Investigator)
3. Crohn's disease in India: A multicenter study from a country where intestinal tuberculosis
as well as Johne’s Disease is endemic (Co-Principal Investigator)
4. Molecular epidemiology and anti tuberculosis drug resistance in HIV positive and
negative children with tuberculosis. (Co-Principal Investigator)
5. Validation of indigenously developed technologies for diagnosis of: (a) pulmonary
tuberculosis (b) drug resistant tuberculosis under Phase 1: Validation of indigenous test
for diagnosis of pulmonary tuberculosis from sputum samples, Phase 2: Validation of
indigenous test for diagnosis of drug resistant tuberculosis from sputum samples (Co-
Investigator)
6. Strengthening of Mycobacterial Repository for translational studies –Phase II. (Co-
Investigator)
Scientific Conferences/ workshop organized
1. Joint- Organizing Secretary, Hands- on Workshop on “Bio-Safety and Bio-Security”
(April 6-10, 2015) at National JALMA Institute for Leprosy and Other Mycobacterial
Diseases, Agra.
2. Organizing Secretary, Joint National workshop on “Good laboratory Practices ”(May 11-
12, 2015) at Department of Biotechnology, Mangalayatan University, Aligarh and
National JALMA Institute for Leprosy and Other Mycobactrial Diseases, Agra
Referee for Research grants
1. Revised National Tuberculosis Control Program (RNTCP) State Operational Research
Programme (Uttar Pradesh).
2. Short Term Studentship (STS) program, Indian Council of Medical Research, New Delhi.
Publications:
Research and Review Articles
1. Singh AV, Chauhan DS, Yadav V, Singh SV. Current Status of Mycobacterium avium
subspecies paratuberculosis infection in animals and humans in India: What needs to be
done? Indian Journal of Medical Research (Accepted).
2. Singh AV. Chauhan DS, Singh A. Mixed infection of bovine tuberculosis and
paratuberculosis in domestic livestock species of North India. International Journal of
Infectious Diseases. (Accepted)
3. Singh P, Katoch VM, Mohanty KK, Singh AV, Chauhan DS. Analysis of expression
profile of mce operon genes (mce1, mce2, mce3 operon) in different Mycobacterium
tuberculosis isolates at different growth phases. Indian Journal of Medical Research
(Accepted).
4. Chauhan DS, Sharma R, Parashar D, Das R, Sharma P, Chahar M, Singh AV,
Singh PK,
Katoch K,
Katoch VM. 2016. Early detection of Multidrug Resistant (MDR)
Mycobacterium tuberculosis in a single tube with in-house designed Fluorescence
resonance energy transfer (FRET) Probes using Real-time PCR. Indian Journal of
Experimental Biology. 54:229-236.
5. Singh, A.V., Chauhan, D.S., Singh, A., Singh, P.K., Sohal, J.S., Singh, S.V., 2014.
Application of IS1311 Locus 2 PCR-REA assay for the specific detection of ‘Bison Type’
Mycobacterium avium subspecies paratuberculosis strains of Indian origin. Indian Journal
of Medical Research. 141(1): 55–61.
6. Garg, R., Patil, P.K., Singh, S.V., Sharma, S., Ravi Kumar, G.V.P.P.S., Singh, A.V.,
Filia,G., Singh, P. K., Jayaraman,S., Gupta, S., Chaubey, K.K., Tiwari, R., Saminathan, M.,
Dhama, K., Sohal, J.S., 2015. Comparative Evaluation of Different Test Combinations for
Diagnosis of Mycobacterium avium subspecies paratuberculosis Infecting Dairy Herds in
India. BioMed Research International. Volume 2015 (2015), Article ID 983978.
(http:dx.doi.org/10.1155/2015/983978)
7. Periwal V, Patowary A, Vellarikkal S, Gupta A, Singh M, Mittal A, Jeyapaul S, Chauhan
R, Singh, AV, Singh PK, Garg P, Katoch VM, Katoch K, OSDD Consortium, Chauhan
DS, Sivasubbu SS, Vinod S. 2015. Comparative whole-Genome Analysis of Clinical
Isolates Reveals Characteristic Architecture of Mycobacterium tuberculosis Pangenome.
PLOS ONE. 8;10(4):e0122979.
8. Singh, S.V., Singh, P.K., Singh, A.V., Sohal, J.S., Kumar, N., Chaubey, K.K., Gupta, S.,
Rawat, K.D., Kumar, A., Bhatia, A.K., Srivastav, A.K., Dhama, K. 2014. Bio-load’ and
bio-type profiles of Mycobacterium avium subspecies paratuberculosis infection in the
domestic livestock population endemic for Johne’s disease: a survey of 28 years (1985-
2013) in India. Transboundary and Emerging Diseases 61 Suppl 1: 43-55.
9. Barad DB, Chandel BS, Dadawala AI, Chauhan HC, Kher HS, Shroff S, Bhagat AG,
Singh SV, Singh PK, Singh AV, Sohal JS, Gupta S, Chaubey KK, Chakraborty S, Tiwari
R, Deb R, Dhama K . 2014. Incidence of Mycobacterium avium subspecies
paratuberculosis in Mehsani and Surti goats of Indian origin using multiple diagnostic
tests. Journal of Biological Sciences, 14 (2): 124-133.
10. Singh, S.V., Kumar, N., Sohal J.S., Singh A.V., Singh P.K., Agrawal N.D., Gupta, S.,
Chaubey K.K., Deb, R., Dhama, K., Rawat K.D., 2014. First mass screening to estimate
bio-load of Mycobacterium avium subspecies paratuberculosis in the human population in
North India. Journal of Public Health and Epidemiology, 6 (1); 20-29.
11. Singh S.V., Kumar N., Sohal J.S., Singh A.V., Singh P.K., Agrawal N.D., Gupta S.,
Chaubey K.K., Rawat K.D., Deb R., Dhama K. 2014. First mass screening of the human
population to estimate the bio-load of Mycobacterium avium subspecies paratuberculosis
in North India. Journal of Biological Sciences (Accepted-In Press).
12. Singh S.V., Singh P.K., Gupta S., Chaubey K.K., Singh B., Kumar A., Singh A.V.,
Kumar N. 2013. Comparison of microscopy and blood-PCR for the diagnosis clinical of
Johne’s disease in domestic ruminant. Iranian Journal of Veterinary Research, 14: 345-
349.
13. Vellarikkal, S., Singh, A.V., Singh, P.K., Garg, P., Katoch, V.M., Katoch, K., Open
Source Drug Discovery Consortium, Chauhan, D.S., Scaria, V., Sivasubbu, S., 2013. Draft
Genome Sequence of an Extensively Drug-Resistant Mycobacterium tuberculosis Clinical
Isolate of the Ural Strain OSDD493. Genome Announcements 2013 Nov 7;1(6).
14. Vellarikkal, S., Singh, A.V., Singh, P.K., Garg, P., Katoch, V.M., Katoch, K., Open
Source Drug Discovery Consortium, Chauhan, D.S., Scaria, V., Sivasubbu, S., 2013. Draft
genome sequence of a multidrug resistant (MDR) clinical isolate of Mycobacterium
tuberculosis belonging to a novel spoligotype. Genome Announcements 2013 Nov
21;1(6).
15. Vellarikkal, S., Singh, A.V., Singh, P.K., Garg, P., Katoch, V.M., Katoch, K., Open
Source Drug Discovery Consortium, Chauhan, D.S., Sivasubbu, S., Scaria, V., 2013. Draft
genome sequence of extensively drug resistant Mycobacterium tuberculosis clinical isolate
OSDD515 belonging to Uganda I genotype. Genome Announcements 2013 Nov 21;1(6).
16. Singh SV, Gupta S, Singh PK, Singh AV, Sohal JS, Kumar N, Kumar A, Chaubey KK,
Singh B. 2013. Therapeutic Management of Clinical Clinical Bovine Johne’s disease
Using Goat Based ‘Indigenous Vaccine’ in Native Hariana Cattle: Case Reports.
Advances in animal and Veterinary Sciences 1 (1S), 23-28.
17. Vellarikkal, S., Patowary, A., Singh, M., Periwal,V., Singh, A.V., Singh, P.K., Garg, P.,
Katoch, V.M., Katoch, K., Jangir, P., Sharma, R., Open Source Drug Discovery
Consortium, Chauhan, D.S., Scaria, V., and Sivasubbu, S., 2013. Draft Genome
Sequence of a Clinical Isolate of Multidrug-Resistant Mycobacterium tuberculosis East
African Indian Strain OSDD271.Genome Announcements, 1(4): e00541-13.
18. Singh,S.V., Kumar, N., Singh, S.N., Bhattacharya,T., Sohal, J.S., Singh,P.K., Singh, A.V.,
Singh,B., Chaubey, K.K., Gupta, S., Sharma, N., Kumar, S., Raghava, G.P.S., 2013.
Genome Sequence of the “Indian Bison Type” Biotype of Mycobacterium avium subsp.
paratuberculosis Strain S5. Genome Announcements 1(1):e00005-13.
19. Singh PK, Singh AV and Chauhan DS. 2013. Current understanding on micro RNAs and
its regulation in response to mycobacterial infections. Journal of Biomedical Sciences.
20:14, doi:10.1186/1423-0127-20-14.
20. Singh PK, Singh SV, Saxena VK, Singh MK, Singh AV and Sohal JS. 2013. Expression
profile of different cytokine genes in peripheral blood mononuclear cells of goats infected
experimentally with native strains of Mycobacterium avium subspecies paratuberculosis.
Animal Biotechnology, 14, 24(3):187-97.
21. Singh SV, Singh AV, Singh PK, Gupta S, Singh H, Singh B, Vinodh Kumar OR,
Rajendiran AS, Swain N, Sohal JS. 2013. Evaluation of ‘Indigenous vaccine’ developed
using ‘Indian Bison Type’ genotype of Mycobacterium avium subspecies paratuberculosis
strain ‘S5’ of goat origin in a sheep flock endemic for Johne’s disease: A three years trial
in India. World Journal of Vaccines. 3, 52-59.
22. Sohal JS, Singh SV, Singh PK, Singh AV, Kumar N, 2013. A new marker IS 1311 L2
PCR-REA for identification of ‘Indian Bison Type’ Mycobacterium avium subspecies
paratuberculosis. Indian J Biotechnology. 12, 204-207.
23. Singh SV, Singh PK, Singh AV, Gupta S, Chaubey KK, Singh B, Kumar A, Srivastav A,
Sohal JS. 2013. Bio-burden and bio-type profiles of Mycobacterium avium subspecies
paratuberculosis infection in suspected population of domestic livestock in India.
International Journal of Current Research, 5:1897-1901.
24. Singh SV, Singh AV, Kumar A, Singh PK, Deb R, Verma AK, Kumar A, Tiwari R,
Chakraborty S, Dhama K. 2013. Survival mechanisms of Mycobacterium avium
subspecies paratuberculosis within host species and in the environment—A review.
Natural Science, 5-6: 710-723.
25. Barad DB, Chandel BS, Dadawala AI, Chauhan HC, Kher HN, Shroff S, Bhagat AG,
Singh SV, Singh PK, Singh AV, Sohal JS, Gupta S, Chaubey KK (2013). Comparative
potential of traditional versus modern diagnostic tests in estimating incidence of caprine
Johne’s disease. Advance in Animal and Veterinary Sciences, 1 (1): 35-40.
26. Singh SV, Chaubey KK, Kumar N, Singh PK, Sohal JS, Gupta S, Singh AV, Verma A,
Dhama K. 2013. Impact of host genetics on susceptibility and resistance to
Mycobacterium avium subspecies paratuberculosis infection in domestic ruminants.
Pakistan Journal of Biological Sciences, 16: 251-266.
27. Singh AV, Chauhan DS, Kumar A, Singh PK and Singh SV. 2012. Potential Etiologic
Link and Association between Mycobacterium avium Subspecies Paratuberculosis and
Crohn’s Disease in Humans. Research & Reviews: A Journal of Immunology 2 (1): 20-33.
28. Singh PK, Singh SV, Singh MK, Saxena VK, Horin P, Singh AV and Sohal JS. 2012.
Effect of genetic variation in the MHC Class II DRB region on resistance and
susceptibility to Johne´s disease in endangered Indian Jamunapari goats. International
Journal of Immunogenetics 39 (4): 314–320.
29. Singh PK, Singh SV, Singh MK, Saxena VK, Singh AV and Sohal JS 2012. Genetic
analysis of MHC Class II DRB gene in an endangered Jamunapari breed of goats. Indian
Journal of Biotechnology 11:220-223.
30. Singh SV, Singh AV, Gupta S, Rajindran AS, Swain N, Singh PK, Singh H, Sohal JS,
Kumar N. 2012. Interspecies Sharing of “Indian Bison Type,” A novel predominant
genotype of Mycobacterium avium subspecies paratuberculosis between naturally
infected and endemic flocks of bharat merino sheep and a colony of rabbits (Oryctolagus
Cuniculus) raised on the same ecosystem in South India. Research and Reviews: A
Journal of Life Sciences, 2(3): 1-8.
31. Singh SV, Tiwari A, Singh AV, Singh PK, Singh B, Kumar A, Gururaj K, Gupta S,
Kumar N. 2012. Contamination of natural resources (soil & river water) with
Mycobacterium avium subspecies paratuberculosis in three districts of Uttar Pradesh : A
Pilot study. Harayana Veterinary Journal, 51:1-5.
32. Singh AV, Singh SV, Singh PK, Sohal JS, Singh MK. 2011. High prevalence of
Mycobacterium avium subspecies paratuberculosis (‘Indian Bison Type’) in animal
attendants suffering complaints who work with goat herds endemic for Johne’s disease in
India. International Journal of Infectious Diseases 15(10):e677-683.
33. Singh AV, Singh SV, Verma DK, Yadav R, Singh PK, Sohal JS. 2011. Evaluation of
Indigenous absorbed ELISA Kit” for the estimation of sero-prevalence of Mycobacterium
avium subspecies paratuberculosis antibodies in human beings in North India. ISRN
Veterinary Science, 2011: 636038, doi:10.5402/2011/636038.
34. Singh SV, Singh AV, Singh PK and Kumar A, 2011. Molecular identification and
characterization of Mycobacterium avium subsp paratuberculosis in free living non-
human primate (Rhesus macaques) from North India. Comparative Immunology
Microbiology and Infectious Diseases 34 (3): 267-271.
35. Singh SV, Singh AV, Singh PK, Singh B, Rajendran AS, Swain N. 2011. Recovery of
Indian Bison type genotype of Mycobacterium avium subsp. paratuberculosis from Wild
Bison (Bos gourus) in India. Veterinary Research 4 (3): 61-65.
36. Singh SV, Singh PK, Singh MK, Singh AV, Sohal JS. 2011. Therapeutic potential of
Johne’s disease vaccine: A follow up post vaccination study in a goatherd of endangered
Jamunapari breed naturally infected with Mycobacterium avium subsp. paratuberculosis.
International Journal of Livestock Production 2 (13): 192-204.
37. Singh SV, Solanki M, Kumar A, Singh PK, Singh AV, Singh B, Sohal JS. 2011.
Comparative evaluation of improved ‘Modified Microscopic Test’ with traditional
microscopy, indigenous ELISA kit, fecal and blood PCR for the diagnosis of
Mycobacterium avium subs paratuberculosis in goatherd endemic for Johne’s disease.
Research & Review: A Journal of Life Sciences 1 (1):8-15.
38. Singh AV, Singh SV, Singh PK and Sohal JS. 2010. Genotype diversity in Indian isolates
of Mycobacterium avium subspecies paratuberculosis recovered from domestic and wild
ruminants from different agro-climatic regions. Comparative Immunology Microbiology
& Infectious Diseases 33: e127–131.
39. Singh AV, Singh SV, Singh PK, Sohal JS 2010. Is Mycobacterium avium subsp
paratuberculosis, the cause of Johne’s disease in animals, a good candidate for Crohn’s
disease in man? Indian Journal of Gastroenterology 29 (2): 53-58.
40. Singh AV, Singh SV, Singh PK and Mahaur K. 2010. Mycobacterium avium subspecies
paratuberculosis as possible cause of Crohn’s disease: An emerging disease of public
helath significance in India. Asian Journal of Experimental Biological Sciences 1 (3): 642-
652.
41. Singh AV, Singh SV, Chauhan A, Singh PK, Sohal JS. 2010. Prevalence estimates and
molecular typing of Mycobacterium avium subspecies Paratuberculosis isolates of buffalo
(Bubalus bubalus) origin from different geographical region of north India. The Indian
Buffalo Journal 8 (1&2): 1-4.
42. Singh AV, Singh SV, Sohal JS, Singh PK. 2010. Genotype profiles of Mycobacterium
avium subspecies paratuberculosis recovered from suspected and Crohn's disease patients
in India. Journal of Communicable Diseases 42(2):91-100.
43. Singh AV, Singh SV, Singh PK, Sohal JS, Mahour K. 2010. Sero-surveillance of
Mycobacterium avium subspecies paratuberculosis infection in domestic livestock in
north India using indigenous absorbed ELISA test. Journal of Advance Laboratory
Research Biology 1: 1-5.
44. Kumar S, Singh SV, Singh AV, Singh PK, Sohal J S and Maitra A, 2010. Wildlife
(Boselaphus tragocamelus) small ruminant (goat and sheep) interface in the transmission
of 'Bison type' genotype of Mycobacterium avium subspecies paratuberculosis in India
Comparative Immunology, Microbiology and Infectious Diseases 33: 145-159.
45. Singh SV, Singh PK, Singh AV, Sohal JS and Sharma, MC, 2010.Therapeutic effects of a
new ‘Indigenous Vaccine’ developed using novel native ‘Indian Bison type’ genotype of
Mycobacterium avium subspecies paratuberculosis for the control of clinical Johne’s
disease in naturally infected goatherds in India. Veterinary Medicine International
2010:351846, doi: 10.4061/2010/351846.
46. Singh, SV, Singh, PK, KumarH.,SohalJ.S.,SinghA.V. 2010. Evaluation of blood PCR
with blood culture, ELISA and microscopic examination for the diagnosis of
Mycobacterium avium subspecies paratuberculosis infection in goat kids. The Indian
Journal of Small Ruminants 16 (1): 67-73.
47. Shankar H, Singh SV, Singh PK, Singh AV, Sohal JS and Greenstein, RJ, 2010. Presence
characterization and genotype profiles of Mycobacterium avium subspecies
paratuberculosis from unpasteurized individual and pooled milk, commercial pasteurized
milk and milk products in India by culture, ELISA, PCR and PCR-REA methods.
International Journal of Infectious Diseases 14: e121-126.
48. Singh PK, Singh SV, Kumar H, Sohal JS and Singh AV, 2010. Diagnostic application of
IS900 PCR using blood as a source sample for the detection of Mycobacterium avium
subspecies paratuberculosis in early and sub-clinical cases of Caprine Paratuberculosis.
Veterinary Medicine International; 2010: 748621. doi: 10.4061/2010/748621.
49. Sohal JS, Singh SV, Singh PK and Singh AV 2009. Strain diversity within
Mycobacterium avium subspecies paratuberculosis: A Review. Indian Journal of
Experimental Biology 48: 7-16.
50. Sohal JS, Singh SV, Singh PK and Singh AV, 2010. On the evolution of 'Indian Bison
type' strains of Mycobacterium avium subspecies paratuberculosis. Microbiological
Research 165:163-171.
51. Raghuvanshi TS, Sharma RB, Singh AV, Singh B, Singh SV, Dhama K. 2010. Indigenous
milk ELISA kit’ vis a vis multiple test regime for the estimation of lacto-prevalence of
Mycobacterium avium subspecies paratuberculosis in goat herds endemic for Johne’s
disease. Indian Journal of Comparative Microbiology Immunology and infectious
Diseases 31:41-43.
52. Singh AV, Singh SV, Singh PK, Sohal JS, Swain N, Rajindran AS, Vinodh OR. 2009.
Multiple tests based prevalence estimates of Mycobacterium avium subspecies
paratuberculosis infection in elite farms of goats and sheep. The Indian Journal of Small
Ruminant 15 (2): 178-182.
53. Singh AV, Singh SV, Sohal JS, Singh PK. 2009. Comparative potential of ‘modified
indigenous’, ‘indigenous’ and ‘commercial’ ELISA kits for the diagnosis of
Mycobacterium avium subspecies paratuberculosis in goat an sheep. Indian Journal of
Experimental Biology 47: 379-382.
54. Singh SV, Singh AV, Singh PK, Sohal JS. 2009. Prevalence of Juvenile Capri
Paratuberculosis and genotyping of Mycobacterium avium subspecies paratuberculosis
recovered from postnatal kids. Indian Journal of Animal Sciences 79 (3): 235-238.
55. Singh SV, Singh AV, Singh PK, Sohal JS. 2009. Prevalence of Juvenile Capri
Paratuberculosis and genotyping of Mycobacterium avium subspecies paratuberculosis
recovered from post natal kids. Indian Journal of Animal Sciences 79 (3): 235-238.
56. Sohal JS, Singh SV, Subhodh S, Sheoran N, Narayansamy K, Singh PK, Singh AV and
Maitra A, 2009. Mycobacterium avium subspecies paratuberculosis diagnosis and geno-
typing: Genomic insights. Microbiological Research 164 (3): 330-337.
57. Singh PK, Singh SV, Singh AV and Sohal JS, 2009. Variability in susceptibility of
different Indian goat breeds with respect to natural and experimental infection of
Mycobacterium avium subspecies paratuberculosis. Indian Journal of Small Ruminant
Research 15(1): 35-43.
58. Singh SV, Sohal JS, Singh PK and Singh AV, 2009. Genotype Profiles of Mycobacterium
avium subspecies paratuberculosis isolates recovered from animals, commercial milk and
human beings in North India. International Journal of Infectious Diseases 13:e221-227.
59. Mishra P, Singh SV, Bhatiya AK, Singh PK, Singh AV, Sohal JS. 2009. Prevalence of
Bovine Johne’s disease (BJD) and Mycobacterium avium subspecies paratuberculosis
genotypes in dairy cattle herds of Mathura district. Indian Journal of Comparative
Microbiology Immunology and Infectious Diseases 30: 23-25.
60. Shisodiya AS, Panwar A, Singh SV, Singh PK, Singh AV, Singh B, Kumar A. 2009.
Prevalence of Mycobacterium avium subspecies paratuberculosis,an animal pathogen, in
the population of animal keepers of Ghaziabad and Saharanpur districts of North India
using multiple diagnostic tests. Indian Journal of Comparative Microbiology Immunology
and Infectious Diseases 30: 42-44.
61. Sohal JS, Subodh S, Singh SV, Singh AV, Singh PK. 2009. Interspecies sharing of
‘Indian Bison type’ Mycobacterium avium subspecies paratuberculosis as revealed by the
‘Short Sequence Repeat Typing’. Indian Journal of Animal Sciences 79 (3): 272-274.
62. Singh AV, Singh SV, Makharia GK, Singh PK, Sohal JS. 2008. Presence and
Characterization of Mycobacterium avium subspecies paratuberculosis from clinical and
suspected cases of Crohn's disease and in healthy human population in India. International
Journal of Infectious Diseases 12: 190-197.
63. Singh SV, Singh AV, Singh R, Sharma S, Shukla N, Misra S, Singh PK, Sohal JS, Kumar
H, Patil P K, Misra P and Sandhu KS, 2008. Sero–prevalence of Johne’s disease in
buffaloes and cattle population of North India using indigenous ELISA kit based on native
Mycobacterium avium subspecies paratuberculosis ‘Bison type’ genotype of goat origin.
Comparative Immunology Microbiology and Infectious Diseases 31 (5): 419-433.
64. Singh SV, Singh AV, Singh PK, Sohal JS. 2008. An outbreak of caprine paratuberculosis
in a newly established commercial goat farm. Haryana Vet 47:110-112.
65. Yadav D, Singh SV, Singh AV, Sevilla I, Juste RA, Singh PK and Sohal JS, 2008.
Pathogenic ‘Bison-type’ Mycobacterium avium subspecies paratuberculosis genotype
characterized from riverine buffalo (Bubalus bubalis) in North India. Comparative
Immunology Microbiology and Infectious Diseases 31: 373–387.
66. Singh PK, Singh SV, Singh AV and Sohal JS, 2008. Caprine paratuberculosis in farm and
farmer’s goatherds: An assessment of prevalence in target tissues, comparison of culture,
PCR and indigenous ELISA kit and genotypes of Mycobacterium avium subspecies
paratuberculosis. Indian Journal of Small Ruminants 14: 211-217.
67. Vohra J, Singh SV, Singh AV, Singh PK, Sohal JS. 2008. Detection and characterization
of Mycobacterium avium subspecies paratuberculosis from the vaginal secretions of post-
parturient farm goats, using culture, IS900 PCR and ELISA kit. Indian Journal of Animal
Sciences 78 (5): 441-445.
68. Vohra J, Singh SV, Singh AV, Singh PK, Sohal JS. 2008. Comparative distribution of
Mycobacterium avium subspecies paratuberculosis in the target and non-target tissues of
goats and sheep population in India. Indian Journal of Animal Sciences 78 (4): 342-347.
69. Singh PK, Singh SV, Singh AV, Sohal JS. 2008. Screening of tissues and serum by
culture, PCR and ELISA for the detection of Mycobacterium avium subspecies
paratuberculosis from cases of clinical ovine Johne’s disease in farmer’s flocks. Indian
Journal of Animal Sciences 78 (10): 1052-1056.
70. Singh PK, Singh SV, Singh AV, Sohal JS, Singh NP. 2008. Evaluation of four methods of
DNA recovery from Mycobacterium avium subspecies paratuberculosis present in
intestinal tissues of goats and comparative sensitivity of IS900 PCR with respect to
culture for the diagnosis of Johne’s disease. Indian Journal of Experimental Biology 46:
579-582.
71. Sharma G, Singh SV, Sevilla I, Singh AV, Whittington RJ, Juste RA, Kumar S, Gupta
VK, Singh PK, Sohal JS and Vihan VS, 2008. Evaluation of indigenous milk ELISA with
m-culture and m-PCR for the diagnosis of Bovine Johne’s disease (BJD) in lactating
Indian dairy cattle. Research in Veterinary Science 84:30-37.
72. Kumar S, Singh SV, Sevilla I, Singh AV, Whittington RJ, Juste RA, Sharma G, Singh PK,
Sohal JS. Lacto-prevalence, genotyping of Mycobacterium avium subspecies
paratuberculosis and evaluation of three diagnostic tests in milk of naturally infected
goatherds. Small Ruminant Research 74 (1-3): 37-44.
73. Shakar, H., Singh, S.V., Singh, P.K., Singh, A.V., Sohal, J.S. 2008. Recovery of live
Mycobacterium avium subspecies Paratuberculosis From milk and milk products from
North India. Indian Journal of Comparative Microbiology, Immunology and Infectious
Diseases 29: 31-35.
74. Sohal JS, Singh SV, Tyagi P, Subodh S, Narayanswamy K, Singh PK and Singh AV,
2008. Immunology of Mycobacterial Infections: With special reference to Mycobacterium
avium subspecies paratuberculosis. Immunobiology 213: 585-598.
75. Singh SV, Singh AV, Singh R, Sandhu K S, Singh PK, Sohal JS, Gupta VK and Vihan
VS, 2007. Evaluation of highly sensitive indigenous milk ELISA kit with fecal culture,
milk culture and fecal-PCR for the diagnosis of bovine Johne’s disease (BJD).
Comparative Immunology Microbiology and Infectious Diseases 30: 175-186.
76. Singh SV, Singh AV, Singh PK, Gupta VK, Kumar S and Vohra J, 2007. Sero-prevalence
of paratuberculosis in young kids using “Bison type’, Mycobacterium avium subsp.
paratuberculosis antigen in plate ELISA. Small Ruminant Research 70:89-92.
77. Singh SV, Singh PK, Singh A V, Sohal J S and Gupta VK. 2007. Comparative efficacy of
an indigenous 'Inactivated vaccine' using highly pathogenic field strain of Mycobacterium
avium subspecies paratuberculosis 'Bison type' with a commercial vaccine for the control
of Capri - Paratuberculosis in India. Vaccine 25: 7102-7110.
78. Singh SV, Singh AV, Singh R, Sandhu KS, Singh PK, Sohal JS. 2007. Comparison of
multiple diagnostic tests for the diagnosis of bovine paratuberculosis in lactating cows.
Indian J of Animal Sciences 77 (11): 1115-1117.
79. Singh SV, Singh AV, Singh PK, Sohal JS, Singh NP. 2007. Evaluation of an indigenous
ELISA for diagnosis of Johne’s disease and its comparison with commercial kits. Indian
Journal of Microbiology 47 (3): 251-258.
80. Singh SV, Singh AV, Sandhu KS, Singh PK, Sohal JS. 2007. Sero-prevalence of Johne’s
disease in bovine breeding bulls using indigenous ELISA kit. Haryana Vet 46: 69-71.
81. Singh SV, Singh AV, Singh PK, Sohal JS, Vikram Y, Kharche SD. 2007. Sero-prevalence
of Mycobacterium avium subspecies paratuberculosis infection in Barbari and Sirohi
breeds of goats in their respective home tracts in semi-arid regions of North India.
Haryana Vet 46: 104- 106.
82. Singh SV, Singh AV, Shukla N, Singh PK, Sohal JS, Gupta VK, Vihan VS. 2007. Sero-
prevalence of Johne’s disease in prospective young bulls of Hariana breed by indigenous
ELISA kit, using protoplasmic antigen from ‘Bison type’ genotype of Mycobacterium
avium subspecies paratuberculosis of goat origin. Indian Journal of Animal Sciences 77
(8): 659-662.
83. Singh SV, Singh AV, Singh R, Misra S, Shukla
N, Singh PK, Sohal JS, Sharma S, Kumar
H, Patil PK, Sandhu KS. 2007. Real time estimates of sero–prevalence of Johne’s disease
in farmer’s and farm goatherds in North India, using indigenous ELISA kit and fecal
culture. Indian Journal of Animal Sciences 77 (11): 1074-1079.
84. Kumar P, Singh S V, Bhatiya AK, Sevilla I, Singh AV, Whittington RJ, Juste RA, Gupta
VK, Singh P K, Sohal JS and Vihan VS, 2007. Juvenile Capri-Paratuberculosis in India;
Incidence and characterization of by 6 diagnostic tests. Small Ruminant Research 73:45-
53.
85. Singh SV, Singh PK, Singh AV, Sohal JS. 2007. Non-chemical method of DNA recovery
for the characterization of Mycobacterium avium subspecies paratuberculosis using IS
900 PCR. Indian Journal of Experimental Biology 45: 812-816.
86. Sohal JS, Singh SV, Subhodh S, Singh AV, Singh PK, Sheoran N, Sandhu KS,
Narayanasamy K and Maitra A. 2007. Mycobacterium avium subspecies paratuberculosis
Diagnosis and Strain Typing: Present Status and Future Developments. Indian Journal of
Experimental Biology 45 (10): 843-852.
87. Mishra P, Singh SV, Bhatiya AK, Singh PK, Singh AV, Sohal JS. 2007. Genotyping of
Mycobacterium avium subspecies paratuberculosis by IS900 PCR and IS 1311 PCR-REA
from dairy cattle herds of Mathura region of North India and estimates of Bovine Johne's
Disease prevalence using sensitive fecal culture and goat based 'Indigenous ELISA kit'.
Indian Journal of Animal Sciences 78 (11): 1186-91.
88. Kumar S, Singh SV, Maitra A, Singh AV, Singh PK, Sohal JS. 2007. Inter-species
transmission of 'Bison type' genotypes of Mycobacterium avium subspecies
paratuberculosis between Boselaphus tragocamelous (Blue bulls) and small ruminant
population in India. Indian Journal of Animal Science 78 (11): 1186-91.
89. Singh SV, Singh AV, Makharia G, Naser SA, Singh PK, Sohal JS, Gupta VK, Vihan VS.
2006. Crohn's disease and Johne's disease: a comparison with reference to national and
international perspective and public health significance. Intas Polivet 7 (2): 275-300.
90. Singh AV, Das R, Gupta P, Sharma VD, Katock VM. 2004. Identification of gene
mutations in rpoB gene of rifampicin resistant isolates of Mycobacterium tuberculosis by
automated DNA sequencing. Journal of Immunology and Immunopathology 6: 94-95.
Books/ Manuals /Book Chapters
1. Singh S.V, Singh PK, Kumar A, Singh AV. 2010. Mycobacterium avium subsp.
paratuberculosis: a predominant biotic stress affecting growth, development and
productivity in domestic ruminants. In: Climate Change & Stress Management: Sheep and
Goat production; Publisher Satish Serial Publishing House, (ISBN-13: 978-8189304812)
2. Singh, S.V., Sohal, J.S., M. C. Sharma, Singh, P.K., Singh, A.V., 2009. Mycobacterium
avium subspecies paratuberculosis infection in animals and public health aspects.
Publisher Director, CIRG, Makhdoom. p. 104.
3. Singh, S.V., Singh, P.K., Singh, A.V., Sohal, J.S., and M.C. Sharma. 2009. Achievements
in paratuberculosis research (1984-2009) Publisher Director, CIRG, Makhdoom. p. 173.
4. Singh, S.V., M. C. Sharma, Sohal, J.S., Singh, A.V., Singh, P.K., 2009. Johne’s disease
status, diagnosis and control: Indian Scenario. Publisher Director, CIRG, Makhdoom. p.
52.
5. Singh, S.V., M.C. Sharma, Sohal, J.S., Singh, A.V., Singh, P.K., 2009. Johne’s disease of
animals and public health significance. Publisher Director, CIRG, Makhdoom. p.17.
6. Singh, S.V., M.C. Sharma, H.A.Tiwari, Singh, P.K., Singh, A.V., Sohal, J.S., 2009.
Johans Rog : Pashu avum Jan swasth mein mahatva. Publisher Director, CIRG,
Makhdoom.p.22.
7. Singh, S.V., Sohal, J.S., M.C.Sharma, Singh, P.K., Singh, A.V., 2009. Laboratory manual
for the diagnosis of Johne’s disease and isolation, identification and characterization of
Mycobacterium avium subspecies paratuberculosis. Publisher Director, CIRG,
Makhdoom. p. 170.
Conference proceeding papers/ Popular articles:
Selected proceeding papers
1. Singh AV, Chauhan DS. 2016. Biosafety practices for mycobacterial disease research with
special reference to tuberculosis. In Proceeding of Colloquium on Mycobacterial Diseases:
International Status & Priorities, Jaipur. p31-32.
2. Singh AV, Chauhan DS. Mixed Infection of bovine tuberculosis and paratuberculosis in
domestic livestock species of North India. In Proceeding of 17
th
International Congress on
Infectious Disease, Hyderabad,p.94 (43.0267).
3. Singh AV, Chauhan DS, Pachouri PK, Shakya DK, Yadav P, Mohanty PS, Yadav VS. 2016.
Bacteriological positive tuberculosis and drug resistance pattern of Mycobacterium tuberculosis
among pediatric patients suspected for pulmonary and extra-pulmonary tuberculosis in North
India. In Proceeding of 70
th
National Conference on Tuberculosis and chest Diseases- 2015,
Lucknow.
4. Singh AV, Chauhan DS. 2014.Validation of IS1311PCR-REA locus 2 PCR-REA assay for the
specific detection of ‘BisonType’ Mycobacterium avium subspecies paratuberculosis strains of
Indian origin. In Proceeding of International conference on future prospects of advancements in
biological sciences, health issues and environmental protection, Lucknow, p.8-9.
5. Singh AV, Chauhan DS. 2012. Presence estimates and genotypes of Mycobacterium avium
subspecies paratuberculosis in dairy cattle herds of Agra, Mathura and Firozabad region of
North India using microscopic examination, direct IS900 PCR and IS1311 PCR-REA
method. In Proceeding of 11
th
International Colloquium of Paratuberculosis, University of
Sydney, Australia. P. 369.
6. Singh AV, Chauhan DS, Chauhan A. 2011. Detection of major mycobacterial pathogens
(Mycobacterium tuberculosis, Mycobacterium bovis and Mycobacterium avium subspecies
paratuberculosis) in feces of domestic livestock species using microscopy and direct PCR.
In Proceeding of I
st
Biennial conference of Indian Academic of Science and Nature at Agra.
7. Singh AV, Singh SV, Singh PK, Sohal JS. 2010. Genotype profiles of Mycobacterium avium
subspecies paratuberculosis recovered from inflammatory bowel disease patients, suspected
Crohn’s disease patients and healthy human beings in India. In Proceeding of 6th annual
conference of association of medical microbiologist-up chapter in MICROCON-2010 at
National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Agra.
P.206.
8. Singh AV, Singh, S.V., Yadav, R. Singh, P.K.,Kumar, A. 2009. MAP- emerging animal-human
pathogen of global concern with special reference ot Indian sub-continent. International
Conference on Biotechnology, 27-28 Nov, 2009. DAV, College, Jalandhar, p. 42-49
9. Singh AV, Singh, S. V., Singh, P. K Sohal, J. S. & Sharma, M.C. 2009. Novel IS900
sequence polymorphisms in Indian isolates of Mycobacterium avium subspecies
paratuberculosis of human and bovine origin. 10
th
International Colloquium of
Paratuberculosis, University of Minnesota, USA, Aug 9 -14, pp42-49.
10. Singh AV, Singh SV, Sohal JS, Singh PK, Sharma MC.2009. Sharing of 'Indian Bison
Type' Mycobacterium avium subspecies paratuberculosis between goatherds endemic for
Johne's disease and their animal attendants. 10
th
International Colloquium of
Paratuberculosis, University of Minnesota, USA, Aug 9 -14, pp42-49.
11. Singh AV, Singh SV, Singh PK, Sohal JS, Sharma MC. 2009. Novel IS900 sequence
polymorphisms in Indian isolates of Mycobacterium avium subspecies paratuberculosis of
human and bovine origin. 10th International Colloquium of Paratuberculosis, University of
Minnesota, USA, Aug 9 -14,p. 175.
12. Singh AV, Singh SV, Sohal JS, Singh PK, Sharma MC. 2009. Sharing of 'Indian Bison
Type' Mycobacterium avium subspecies paratuberculosis between goatherds endemic for
Johne's disease and their animal attendants. 10th International Colloquium of
Paratuberculosis, University of Minnesota, USA, Aug 9 -14,p. 192.
13. Singh AV, Singh PK, Sohal JS, Singh SV, Malik SVS. 2007. Sero-prevalence of
Mycobacterium avium subspecies paratuberculosis in spontaneous cases of abortions in human
beings in North India using native and commercial protoplasmic antigens in indigenous ELISA
kit. In Proc: 9
th
International Colloquium on Paratuberculosis, JAPAN, p. 332-335.
Conferences / Workshops/ training attended
1. Colloquium on Mycobacterial Diseases: International Status & Priorities held at Amity
University, Jaipur from 12-13 April, 2016.
2. 17
th
International Congress on Infectious Disease, held at Hyderabad International
Convention Centre, Hyderabad from March 2-5, 2016.
3. 70
th
National Conference on Tuberculosis and chest Diseases- 2015 held at Department of
Respiratory Medicine, King George’s Medical University, Lucknow from February 20-21,
2016.
4. International conference on future prospects of advancements in biological sciences,
health issues and environmental protection held at Lucknow from February07-08, 2014.
5. 11th International Colloquium on Paratuberculosis hed at University of Sydney, Sydney,
Australia from February 5–10, 2012.
6. 6th annual conference of association of medical microbiologist-up chapter in
MICROCON-2010 at National JALMA Institute for Leprosy and Other Mycobacterial
Diseases (ICMR), Agra, March 13-14, 2010.
7. National Seminar on Rural India Development Alternatives: Sectoral Convergence for
Livelihood Security held at Central Institute for Research on Goats, Makhdoom, Mathura,
January 16-18, 2009.
8. International Colloquium on Biotechnology at DAV College, Jalandhar, India. November
27-28, 2009.
9. 9th International Colloquium on Paratuberculosis held at Tsukuba, Japan from October 28
– November 2, 2007.
10. National seminar on Emerging diseases of Small ruminant and there containment under
WTO Regime, at Central Institute for Research on Goats, Makhdoom, Mathura, February
3-5,2007.
11. National seminar and workshop on Johne‘s disease held at Division of Pathology, Indian
Veterinary Research institute, Izatnagar from August 8-9, 2007.
12. National seminar on Emerging diseases of small ruminants and their containment under
WTO regime held at Central Institute for Research on Goats, Makhdoom, Mathura from
February3-5, 2007.
13. VIII National Conference of Animal Genetics and breeding on National livestock breeding
policy held at Central Institute for research on Goats, Makhdoom, Mathura from March 8-
10, 2005.
14. Third convention of Society of Immunology and Immuno Pathology (SIIP) and National
symposium on Cytokine and Signal Transduction hed at Central JALMA Institite for
Leprosy and Other Mycobacterial Diseases, Agra from February13-14, 2004.
15. Workshop on Tuberculosis (Modern diagnostics, RNTCP, MDR, KDRTB) at at
Department of Respiratory Medicine, King George’s Medical University, Lucknow on
February 19, 2016.
16. CB-NAAT workshop from November 26-27,2015 at RNTCP at STDC, Agra
17. Hands-on training on “Laboratory diagnosis of Influenza infection (Swine flu)” from
February 2-5, 2015 at National Institute of Virology, Pune
18. Cold chain management awareness course organized by World Courier on November
25,2014 at NIV, Pune.
19. Training on dangerous good management by air organized by World Courier on
November 25, 2014 at NIV, Pune
20. Workshop on “Preparedness for laboratory diagnosis of Ebola virus from November 25-
30, 2014 at National Institute of Virology, Pune.
21. Workshop on “How to write a research proposal” from July 9-11, 2014 at National
JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra
22. CME on Newer molecular tools for the diagnosis and management of tuberculosis and
other mycobacterial Diseases from March 13-14, 2010 at National JALMA Institute for
Leprosy and Other Mycobacterial Diseases, Agra
23. Workshop on Johne’s disease from August 8-9, 2007 at Indian Association of Veterinary
Pathologist at IVRI, Izatnagar
24. Training on Isolation, Immuno-diagnosis and characterization of Mycobacterium avium
subspecies paratuberculosis from December 9-29, 2004 at Central Institute for Research
on Goats, Makhdoom, Farah, Mathura
25. Workshop on “Molecular and immunological methods” on February 15, 2004 at Central
JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra.
Genome / sequences submitted to GenBank Database: (Whole genome sequence-4;
Nucleotide sequence -81)
Whole genome sequence-5
1. Whole Genome Sequence of “Indian Bison Type” Biotype of Mycobacterium
aviumsubsp. paratuberculosis Strain S5 (Accession no.ANPD00000000).
2. Genome sequence of a multi-drug resistant Mycobacterium tuberculosis East African
Indian (EAI) clinical isolate OSDD271 (Accession no. AQQC00000000).
3. Genome sequence of extensively drug resistant Mycobacterium tuberculosis clinical
isolate OSDD515 belonging to Uganda I genotype. (GenomeA00750-13)
4. Genome sequence of an extensively drug-resistant Mycobacterium tuberculosis clinical
isolate OSDD493 of Ural genotype (GenomeA00928-13)
5. Genome sequence of a Multidrug-Resistant clinical isolate of Mycobacterium tuberculosis
belonging to a Novel Spoligotype (Accession AUXD00000000.)
Nucleotide sequence-81
A total of 81 nucleotide sequences (belongs to different locus in the genome of
Mycobacterium avium subspecies paratuberculosis) has been submitted to GenBank
database (NCBI). List were as follow-
I. “Isolation of Mycobacterium avium subsp. paratuberculosis from pasteurized and
unpasteurized milk in north India authored by Singh, A. V., Hermon-Taylor, J., Bull,
T., Singh,S. V., Shankar, H., Singh, P. K. and Sohal, J. S. (2006-07).
S.
No.
Accessed Loci/strain
Accession
Number
S.
No.
Accession
Number
1
IS900- AV1 Region/MAP C8
EU232747
7
EU232753
2
IS900 - AV2 Region/MAP C8
EU232748
8
EU232754
3
IS900 - AV1 Region/MAP C10
EU232749
9
EU232755
4
IS900 - AV2 Region/MAP C10
EU232750
10
EU232756
5
IS900 - AV1 Region/MAP C16
EU232751
11
EU232757
6
IS900 - AV2 Region/MAP C16
EU232752
II. “Genetic Characterization of native isolate of Mycobacterium avium subsp.
paratuberculosis 'Bison type' S5 strain” authored by Sohal, J. S., Subodh, S.,
Brahmachari,V., Sheoran, N., Sandhu, K. S., Singh, P. K., Singh, A.V., et al ., (2005-
2007).
S. No.
Accessed Loci
Accession Number
S. No.
Accessed Loci
Accession Number
1
IS900 Locus 1
EF514818
22
IS1311 Locus 6
EF514839
2
IS900 Locus 2
EF514819
23
IS1311 Locus 7
EF514840
3
IS900 Locus 3
EF514820
24
ISMAP02 Locus 1
EF514841
4
IS900 Locus 4
EF514821
25
ISMAP02 Locus 2
EF514842
5
IS900 Locus 5
EF514822
26
ISMAP02 Locus 3
EF514843
6
IS900 Locus 7
EF514823
27
ISMAP02 Locus 4
EF514844
7
IS900 Locus 8
EF514824
28
ISMAP02 Locus 5
EF514845
8
IS900 Locus 9
EF514825
29
ISMAP02 Locus 6
EF514846
9
IS900 Locus 10
EF514826
30
G Repeat SSR
EF514847
10
IS900 Locus 11
EF514827
31
GGT Repeat SSR
EF514865
11
IS900 Locus 12
EF514828
32
LSP2 (5’ End)
EF514866
12
IS900 Locus 14
EF514829
33
LSP2 (3’ End)
EF514867
13
IS900 Locus 15
EF514830
34
LSP4 (5’ End)
EF514868
14
IS900 Locus 16
EF514831
35
LSP4 (3’ End)
EF514869
15
IS900 Locus 17
EF514832
36
LSP12 (5’ End)
EF514870
16
IS900 Locus 13
EF514833
37
LSP12 (3’ End)
EF514871
17
IS1311 Locus 1
EF514834
38
LSP15 (5’ End)
EF514872
18
IS1311 Locus 2
EF514835
39
LSP15 (3’ End)
EF514873
19
IS1311 Locus 3
EF514836
40
LSP
A
20 (5’ End)
EF514874
20
IS1311 Locus 4
EF514837
41
LSP
A
20 (3’ End)
EF514875
21
IS1311 Locus 5
EF514838
42
LSP
A
4II g
EF514876
III. Molecular Sub-typing of Indian isolates of Mycobacterium avium subsp. paratuberculosis
authored by Sohal, J. S., ubodh, S., Brahmachari,V., Sheoran, N., Sandhu, K. S., Singh, P. K.,
Singh, A.V., et al (2005-2007)
S.
No.
Accessed Loci/strain
Accession
Number
S. No.
Accessed Loci
Accession
Number
1
G Repeat SSR, MAP S4
EF514848
9
GGT Repeat SSR, MAP B42
EF514856
2
G Repeat SSR, MAP 120
EF514849
10
GGT Repeat SSR, MAP S4
EF514857
3
G Repeat SSR, MAP 1090
EF514850
11
GGT Repeat SSR, MAP S12
EF514858
4
G Repeat SSR, MAP 121
EF514851
12
GGT Repeat SSR, MAP 120
EF514859
5
G Repeat SSR, MAP A35
EF514852
13
GGT Repeat SSR, MAP 121
EF514860
6
G Repeat SSR, MAP C43
EF514853
14
GGT Repeat SSR, MAP A35
EF514861
7
G Repeat SSR, MAP 971845
EF514854
15
GGT Repeat SSR, MAP 1090
EF514862
8
G Repeat SSR, MAP 981507
EF514855
16
GGT Repeat SSR, MAP 981507
EF514863
17
GGT Repeat SSR, MAP 971845
EF514864
IV. “Isolation and molecular characterization of Mycobacterium avium subsp. paratuberculosis
from blue bulls authored by Singh,S. V., Maitra, A., Kumar, S., Singh,A.V., Singh,P. K. and
Sohal,J.S. (2005-06)
S.
No.
Accessed Loci/Strain
Accession Number*
1
IS900 Partial Sequence/MAP B42
EU130943
V. “Search of unique Molecular Signature for Indian isolates of Mycobacterium avium subsp.
paratuberculosis authored by Sohal, J. S., Subodh, S., Brahmachari,V., Sheoran, N., Sandhu, K.
S., Singh, P. K., Singh, A. V., Singh, S. V. and Narayanasamy, K. (2008).
S.
No.
Accessed Loci/strain
Accession
Number
S.
No.
Accessed Loci
Accession
Number
1
IS1311 Locus 2/ MAP S12
EU370554
6
IS1311 Locus 5/ MAP S12
EU370559
2
IS1311 Locus 2 / Portugal MAP
1090
EU370555
7
IS1311 Locus 5/ MAP 121
EU370560
3
IS1311 Locus 2/ MAP 121
EU370556
8
IS1311 Locus 5/ MAP 120
EU370561
4
IS1311 Locus 2/ MAP 120
EU370557
9
IS1311 Locus 2/ Portugal
MAP 1090
EU370562
5
IS1311 Locus 5/ MAP S4
EU370558
10
IS1311 Locus 2/ MAP B42
EU370563
VI. “Molecular Characterization of MHC II DRB Region of Indian Jamunapari Goat” authored
by Singh,P.K., Singh,S.V., Kumar,A., Sohal,J.S., Singh,A.V. and Singh,M.K. 2010.
S.
No.
Accessed Loci/strain
Accession Number
1
MHC class II DRB second exon
1382908
Foreign visits
1. National Institute of Animal Health, Japan for the participation in 9
th
International
Colloquium on Paratuberculosis held from 29
th
Oct. to 2
th
Nov., 2007 at Tsukuba, Science
City, Japan
2. The University of Sydney, Australia for the participation in 11
th
International Colloquium
on Paratuberculosis held from February 4-11, 2012 at Sydney, Australia
Student supervised
1. M.Sc. (Biotechnology) 4
2. M.Sc. (Microbiology) 2
3. M.Tech (Biotechnology) 1
(Ajay Vir Singh)