Bio-Data
Name : Beenu Joshi
Date of Birth : 1
st
August 1956
Nationality : Indian
Address for Correspondence: National JALMA Institute for Leprosy and other
Mycobacterial diseases, (ICMR) Taj Ganj, Agra 282001
Telephone / Fax/ email: 0562-2331751-54, 0562-2331756, [email protected]
Educational Qualifications
Ph.D (Zoology) Utkal University 1988
M.Sc.(Zoology) Kumaon University 1978 I
st
Div
B.Sc ( Bot, Zool,Chem) Kumaon University 1975 IInd Div
Ph.D Thesis Title: Immune response in humans with Wuchereria bancrofti infection;
dissection of humoral and cellular responses against parasite antigens.
Research Experience
July 1980-Dec 1984 : JRF, SRF in ICMR project entitled “Studies on T and B
lymphocytes in the etiopathogenesis of Bronchial asthma”
at Medicine Deptt, AIIMS, New Delhi
Jan 1985- Aug 1987 : SRF at Regional Medical Research Centre, Bhubaneswar,
Orissa.
Aug 1988 - April 1989 : Research Officer in ICMR project entitled
“Establishment of NonA-NonB Hepatitis virus specific B
cell/ Hybrid b cell clones” in the Deptt of Pathology,
AIIMS, New Delhi.
April 1989- April 1995 : Research Officer RMRC Jabalpur and Central JALMA
Institute for leprosy, Agra.
April 1995- April 2001 : Senior Research Officer at Central JALMA institute for
Leprosy, Agra.
April 2001–April 2007 :Assistant Director at National JALMA institute for leprosy
Agra.
April 2007- September 2012 : Scientist E at National JALMA institute for leprosy &
OMD, Agra.
Sept 2012- Till date : Scientist F at National JALMA institute for leprosy &
OMD, Agra.
Area of Research
Immunology of Infectious Diseases (Leprosy and tuberculosis)
M. leprae mediated T cell signaling in leprosy and TB. Role of T regulatory cells in
leprosy. Th1/2 cell regulation in leprosy and role of Notch in T cell regulation.
New antigens for the diagnosis and protection in TB, T cell activation in TB.
Foreign visit
1. Visited laboratory of Dr. N.A.Khan at France as visiting associate professor
from 2004-04-01 to 2004-05-31.
2. Visited Laboratory of Prof. N.A. KHAN at France from 25-10- 2001 to 24-11-
2001 and learnt Calcium signalling.
3. Visited Laboratory of Dr. Ottenhoff, University Hospital Building, Leiden 1
st
June 1996-29
th
June 1996 for training on T cell lines and T cell cloning.
4. Invited for International Congress of antibodies-2010 in Bejing, China and
presented paper “ March 25
th
-
Publications
1. Bhavyata Dua, Bhawna Sharma, Joy Kumar Chakma, Mamta Arora, Rekha
Bhadauria, Dipendra Kumar Mitra, Beenu Joshi
.
Expansion of T regulatory cells
in lepromatous leprosy is mediated by Phenolic glycolipid-1. Accepted in Journal
of Medical Microbiology & Diagnosis.
2. Rakesh Patel, Rajni Upadhyay, Bharat Bajaj, V.S.Yadav, Nalini Srivastava,
D.S.Chauhan, Santosh K Kar and Beenu Joshi (2016) Differential Cytokine
Response and IgG Subclasses in Pulmonary TB Patients, household Contacts and
BCG Vaccinated PPD-ve Healthy Controls. Mycobacterial Diseases 6:1
3. Soumi Sadhu, Binod Kumar Khaitan, Beenu Joshi, Utpal Sengupta, Arvind
Kumar Nautiyal and Dipendra Kumar Mitra.Reciprocity between regulatory T
cells and Th17 cells: relevance to polarized immunity in leprosy. Accepted in
PLOS Neglected Tropical Diseases.
4. Gavish Kumar, Neha Sharma, Pushpa Gupta, Beenu Joshi, Umesh Datta Gupta,
Gregor Cevc, Amla Chopra (2016) Improved protection against tuberculosis after
boosting the BCG-primed mice with subunit Ag85a delivered through intact skin
with deformable vesicles. European Journal of Pharmaceutical Sciences 82: 11–
20
5. Sharma, D., Kumar, B., Lata, M., Joshi, B., Venkatesan, K., Shukla, S. and Bisht
D. (2015) Comparative proteomic analysis of aminoglycosides resistant and
susceptible Mycobacterium tuberculosis clinical isolates for exploring potential
drug targets (Accepted Plos One).
6. Sharma B, Upadhyay R, Dua B, Khan NA, Katoch VM, Bajaj B, Joshi B (2015)
Mycobacterium tuberculosis secretory proteins downregulate T cell activation by
interfering with proximal and downstream T cell signalling events. BMC
Immunol 16: 67.
7. Dua B, Dagur PK, Mohanty
KK, Natarajan
M, Sengupta U, Bhadauria R, Joshi B
(2014).
Lymphoproliferative and cytokine response of peripheral blood
mononuclear cells from healthy individuals induced by different antigens of
Mycobacterium leprae. Accepted in Current trends in Biomedical and Life
Sciences.
8. Lagrange PH, Thangaraj SK, Dayal R, Deshpande A, Ganguly NK, Girardi E,
Joshi B, Katoch K, Katoch VM, Kumar M, Lakshmi V, Leportier M, Longuet C,
Malladi SV, Mukerjee D, Nair D, Raja A, Raman B, Rodrigues C, Sharma P,
Singh A, Singh S, Sodha A, Kabeer BS, Vernet G, Goletti D. (2014) A toolbox
for tuberculosis (TB) diagnosis: an Indian multi-centric study (2006-2008);
evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens
for TB diagnosis. PLoS One ;9(5):e96367
9. Farazuddin M, Dua B, Zia Q, Khan AA, Joshi B, Owais M (2014)
Chemotherapeutic potential of curcumin-bearing microcells against hepatocellular
carcinoma in model animals. International Journal of Nanomedicine 9:1139-1152
10. Lagrange PH, Thangaraj SK, Dayal R, Despande A, Ganguly NK, Girardi E,
Joshi B, Katoch K, Katoch VM, Kumar M, Lakshmi V, Leportier M, Longuet C,
Malladi SV, Mukerjee D, Nair D, Raja A, Raman B, Rodrigues C, Sharma P,
Singh A, Singh S, Sodha A, Kabeer BS, Vernet G, Goletti D (2013) A Toolbox
for Tuberculosis (TB) Diagnosis: An Indian Multicentric Study (2006–2008).
Evaluation of QuantiFERON-TB Gold in Tube for TB Diagnosis. PLoS one 8:
e73579
11. Dagur PK, Sharma B, Upadhyay R, Dua B, Rizvi A, Khan NA, Katoch VM,
Sengupta U, Joshi B. (2012) Phenolic-glycolipid-1 and lipoarabinomannan
preferentially modulate TCR- and CD28-triggered proximal biochemical events,
leading to T-cell unresponsiveness in mycobacterial diseases. Lipids Health Dis.
11:119. 38.
12. Kumar G, Shankar H, Chahar M, Sharma P, Yadav VS, Chauhan DS, Katoch
VM, Joshi B. (2012) Whole cell & culture filtrate proteins from prevalent
genotypes of Mycobacterium tuberculosis provoke better antibody & T cell
response than laboratory strain H 37 Rv. Indian J Med Res 135:745-55.
13. Lagrange PH, Thangaraj SK, Dayal R, Despande A, Ganguly NK, Girardi E,
Joshi B, Katoch K, Katoch VM, Kumar M, Lakshmi V, Leportier M, Longuet C,
Malladi SV, Mukerjee D, Nair D, Raja A, Raman B, Rodrigues C, Sharma P,
Singh A, Singh S, Sodha A, Kabeer BS, Vernet G, Goletti D.(2012) A toolbox for
tuberculosis diagnosis: an Indian multicentric study (2006-2008): microbiological
results. PLoS One. 7:e43739.
14. Farazuddin M., Sharma B., Khan A.A., Joshi B., Owais M. (2012) Anticancer
efficacy of perillyl alcohol-bearing PLGA microparticles. Int J Nano Med. 7: 35-
47.
15. Singhal N, Sharma P, Kumar M, Joshi B, Bisht D. (2012) Analysis of intracellular
expressed proteins of Mycobacterium tuberculosis clinical isolates. Proteome Sci.
2012 Mar 1;10(1):14.
16. Dayal R, Verma V, Sharma B, Kumar G, Kumar N, Gupta R, Katoch VM, Joshi
B, Chauhan DS. (2012) Diagnostic value of interferon- gamma release assays
(QuantiFERON-TB Gold® In Tube) in childhood tuberculosis. Indian J Pediatr.
79:183-7.
17. Chaturvedi R., Bansal K., Narayana Y., Kapoor N., Sukumar N.,
Togarsimalemath S.K., Chandra N., Misra S., Ajitkumar P., Joshi B., Katoch
V.M., Patil S.A and Balaji K.N. (2010) The multifunctional PE_PGRS11 protein
from Mycobacterium tuberculosis plays a role in regulating resistance to oxidative
stress. J.Biol Chem 285: 30389-30403.
18. Dagur P.K., Sharma B., Kumar G., Khan N.A., Katoch V.M., Sengupta U. and
Joshi B. (2009) Mycobacterial antigen(s) induce anergy by altering TCR- and
CD28-induced signaling events: insights into T-cell unresponsiveness in Leprosy.
Mol Immunol 47: 943-52.
19. Kumar G., Shankar H., Bisht D., Sharma P., Singhal N., Katoch V.M. and Joshi
B. (2010) A simple and rapid method of sample preparation from culture filtrate
of M. tuberculosis for two dimensional gel electrophoresis. Braz J microbial
41:295-299.
20. Singhal N., Bisht D. and Joshi B. (2009) Immunoprophylaxis of tuberculosis: An
update of emerging trends. Arch Immunol Ther Arch 58: 97-106.
21. Kumar G, Dagur P. K., Singh P.K, Yadav V. S., Katoch V. M., Bajaj B. Gupta R.,
Sengupta U. and Joshi B (2010) Serodiagnostic efficacy of M. tuberculosis 30-32
kDa mycolyl transferases complex for patients with active tuberculosis. Arch
Immunol Ther Exp 58: 57-65.
22. Mitra D.K., Joshi B., Dinda A. K., Rai A., Girdhar B.K., Katoch K., Bindra M.
and Sengupta U (2009) Induction of lepromin reactivity in cured lepromatous
leprosy patients: impaired chemokine response dissociates protective immunity
from delayed type hypersensitivity. Microbes and Infection 11: 1122-1130.
23. Dayal R., Singh A., Katoch V.M., Joshi B., Chauhan D.S., Singh p., Kumar G.
and V.D.Sharma (2008) Serological diagnosis of tuberculosis. Indian J Paed 75:
1219.
24. Kumar G, Dagur P. K., Singh M., Yadav V. S., Dayal R., Singh H. B., Katoch V.
M., Sengupta U. and Joshi B. (2008) Diagnostic Potential of Ag85C in
Comparison to Various Secretory Antigens for Childhood Tuberculosis. Scand.
J.Immunol 68: 177-183.
25. Srinivas M., Rajakumari S., Narayan Y, Joshi B., Katoch V.M., Rajasekharan R.,
Balaji K.N. Functional Characterisation of the phospholipase C activity of
Rv3487c and its localization on the cell wall of Mycobacterium tuberculosis. J
Bioscience 33: 221.
26. Mishra K.C., de Chastelier C., Narayana Y., Bifani P., Brown A.K., Besra G.S.,
Katoch V.M., Joshi,B., Balaji K.N. and Kremer L.(2008) Functional role of PE
domain oand immunogenecity of the Mycobacterium tuberculosis triglycerol
hydrolase Lip Y_. Infect Immun 76: 127.
27. Narayana Y., Joshi B., Katoch V.M., Mishra K.C. and Balaji K.N. (2007)
Differential B-cell responses are induced by Mycobacterium tuberculosis PE
antigens Rv1169c, Rv0978c and Rv1818c. Clinical and Vaccine Imunol 14:1334.
28. Dayal R., Sirohi G., Singh M.K., Mathur P.P., Agarwal B.M., Katoch V.M., Joshi
B., Singh P. and Singh H.B. (2006). Diagnostic value of ELISA serological Tests
in childhood tuberculosis. J Trop Pediatr 52: 433.
29. Joshi B., Khedouci S., Dagur P.K., Hichami A., Sengupta U. and Khan N.A.
(2006) On cell signalling mechanism of Mycobaterium leprae soluble antigen
(MLSA) in Jurkat T cells. Mol Cell Biochem 287:157.
30. Mohanty K.K., Joshi B., Katoch K., Sengupta U. (2004) Leprosy reactions:
Humoral and cellular responses to M.leprae, 65 kDa, 28 kDa and 18 kDa
antigens. Int J Lep 72 :149.
31. Nayak, S., Verma, R., Joshi, B. and Venkatesan, K. (2003) Evaluation of lipids as
antigens by enzyme-linked immunosorbent assay (ELISA) against
Mycobacterium bovis. Indian J Anim Sci 73: 471.
32. Nayak, S., Verma, R., Joshi, B. and Venkatesan, K. (2002) Evaluation of an
enzyme linked immunosorbent assay (ELISA) using phenolic glycolipid (PGL)
and crude antigens for screening of sera of cattle infected with Mycobacterium
bovis. Indian J Anim Sci 72: 627.
33. Hichami, A., Joshi, B., Simonin, A.M. and Khan, N.A. (2002) Role of three
isoforms of phospholipase A2 in capacitative calcium influx in human T cells.
Eur J Biochem 269:5557.
34. Nayak, S., Verma, R., Joshi, B. and Venkatesan, K. (2001) Aspects of humoral
and cell mediated immune response incalves experimentally infected with
M.bovis strains. Indian J Anim Sci 71(8):726.
35. Joshi, B., Girdhar, B.K., Mohanty, K.K. Beuria, M.K., Girdhar, A. and Sengupta,
U. (2001) Immunological profile of long term treated smear negative (LTTSN)
lepromatous patients to M.leprae antigens. Int J Lep 69(3) :195-203.
36. Beuria, M.K., Parkash, Om, Joshi, B., Mohanty, K.K., Katoch, K. and
Sengupta,U. (1998) Effect of chemotherapy on IgG subclasses in the spectrum of
leprosy. Int Arch Allergy Appl Immunol 115: 61.
37. Joshi, B., Goverdhini, P., Chakma, T. and Tiwary, R.S. (1997) Humoral and
Cellular Immune responses in filariasis infections in an Endemic area in central
India. Indian J commun Med Vol. 22: 117.
38. Gyan Chand, Tiwary, R.S. and Joshi, B. (1994) Prevalence of Wuchererria
bancrofti Infection in Rural publication of Distt. Panna, M.P. An Entomological
and parasitological observation. Indian J Prev Soc Med 25: 70.
39. Goverdhini, P., Gyan Chand and Joshi, B. (1994) Preliminary findings from an
Epidemiological study of Bancroftian Filariasis in Panna Distt. M.P. Indian J
Commun Med XIX (1) 7.
40. Kar, S.K., Nayak, A.R., Joshi, B. and Singh, Vinod. (1992) Determinant analysis
and interaction studies on monoclonal antibodies to bovine IgG1 and IgG2. Indian
J Biochem Biophys 29: 25.
41. Dash, S., Rao, K.V.S., Joshi, B., Nayak, N, C. and Panda, S.K. (1991)
Significance of natural polymerized albumin and its receptor in hepatitis B
infection of hepatocytes. Hepatology 13(1): 134.