Curriculum Vitae
1. Name Dr. Deepa Bisht
2. Designation Scientist ‘E’
3. Contact address Department of Biochemistry
National JALMA Institute for Leprosy
and Other Mycobacterial Diseases
(ICMR), Tajganj, Agra 282004
Tele: (0562) 2331751-54 Ext. 226
Fax: (0562) 2331755
4. Date of birth 01-04-1968
5. Area of specialization Biotechnology
6. Subject area of research work Mycobacterial Proteomics and Drug
Delivery Systems.
We are involved in understanding the
resistance mechanism of
aminoglycosides resistant
Mycobacterium tuberculosis clinical
isolates by proteomics approaches.
7. Educational qualifications
M.Sc. (Biochemistry) G.B.Pant Univ. of Agri.& Tech., Pantnagar.
Ph.D. (Biotechnology) Panjab Univ. (Institute of Microbial Technology) Chandigarh.
8. List of publications
1. Mehrotra, J., Bisht, D., Tiwari, V.D. and Sinha, S. (1995) Serological distinction of
Integral plasma membrane proteins as a class of mycobacterial antigens and their
relevance for human T cell activation. Clinical and Experimental Immunology 102: 626-
634.
2. Bisht, D., Mehrotra, J., Dhindsa, M. S., Singh, N. B. and Sinha, S. (1996) A major T-
cell inducing cytosolic 23kDa protein antigen of the vaccine candidate Mycobacterum
habana is superoxide dismutase. Microbiology 142: 1375-1383.
3. Rana, S., Bisht, D and Chakraborti, P. K. (1998) Activation of rat androgen receptor by
androgenic ligands is unaffected by antiandrogens in Saccharomyces cerevisiae. Gene
209: 247-254.
4. Rana, S., Bisht, D. and Chakraborti, P. K. (1999) Synergistic activation of yeast–
expressed rat androgen receptor by modulators of protein kinase-A. Journal of
Molecular Biology 286: 669-681.
5. Owais, M., Masood, A. K., Agrewala, J. N., Bisht, D. and Gupta, C.M. (2001) Use of
liposomes as an immunopotentiating delivery system: in perspective of vaccine vaccine
develpoment. Scandinavian Journal of Immunology 54: 125-132.
6. Bisht, D. (2002) Liposomes as immunoadjuvants. CJIL Quarterly Bulletin XXII: 1-11.
7. Venkatesan, K., Katoch, K., Natrajan, M., Deo, N. and Bisht, D. (2004) Biochemical
markers in leprosy reactions. In Proceedings of 3
rd
convention of Society for
Immunology and Immunopathology held at Agra, 13-15 February. Journal of
Immunology and Immunopathology 6: S 45.
8. Ahmad, N., Alam, M. K., Shehbaz, A., Khan, A., Mannan, A., Hakim, S. Rashid.,
Bisht, D. and Owais, M. (2005) Antimicrobial activity of clove oil and its potential in
the treatment of vaginal candidiasis. Journal of Drug Targeting 13: 555-561.
9. Bisht, D., Owais, M. and Venkatesan, K. (2006) Potential of plant derived products in
the treatment of mycobacterial infections. In: Modern Phytomedicine (Eds. Ahmad,
Aqil, Owais) published by published by WILEY-VCH Verlag Gmbh & Co. KGaA,
Weinheim, Germany. pp: 293-311.
10. Bisht D, Singhal N, Sharma P and Venkatesan K (2006). Analysis of mycobacterial
strains by two-dimensional gel electrophoresis. J Commun Dis 38 (3): 255-262.
11. Bisht, D., Singhal, N., Sharma, P. and Venkatesan, K. (2007) An improved sample
preparation method for analyzing mycobacterial proteins in two-dimensional gels.
Biochemistry (Moscow) 72, 672-674.
12. Venkatesan, K., Bisht, D. and Owais, M. (2008) Accumulation of drugs in tissues. In:
Preclinical development handbook (Edited by Shayne Cox Gad) published by John
Wiley & Sons, pp 429-453.
13. Khan, A., Mallick, AI., Maroof, A., Khan, S, Khan, U., Bisht, D and Owais, M. (2009)
Applications of whey in Biotechnology. In: Advances in cheese whey utilization. (Edited
by Ma Esperanza Cerdán, Ma Isabel González-Siso and Manuel Becerra) published by
WILEY-VCH, Germany, pp 1-34.
14. Qamar, Z., Fatima, N., Alam, M., Bisht, D., Sharma, P., Ahmed, I., Farrukh, A and
Owais, M. (2010) Immunomodulators: Potential in treatment of systemic fungal
infections. In: Combating fungal infections: Problems and remedy. Published by
Springer, Germany, pp 397-422.
15. Kumar, G., Shankar, H., Bisht, D., Sharma, P., Singhal, N., Katoch, V.M. and Joshi, B.
(2010) A simple and rapid method of sample preparation from culture filtrate of M.
tuberculosis for two-dimensional gel electrophoresis. Brazilian Journal of Microbiology
41: 295-299.
16. Kumar, G., Sharma, P., Rathore, G., Bisht, D. and Sengupta, U. (2010) Proteomic
analysis of outer membrane protein of Edwardsiella tarda. Journal of Applied
Microbiology 108: 2214-2221.
17. Sharma, P., Kumar, B., Singhal, N., Katoch, V.M., Venkatesan, K., Chauhan, D.S. and
Bisht, D. (2010) Streptomycin induced protein expression analysis in Mycobacterium
tuberculosis by two-dimensional gel electrophoresis and mass spectrometry. Indian
Journal of Medical Research 132: 400-408.
18. Sharma, P., Kumar, B., Gupta, Y., Singhal, N., Katoch, V.M., Venkatesan, K.
and Bisht,
D. (2010) Proteomic analysis of streptomycin resistant and sensitive clinical isolates of
Mycobacterium tuberculosis. Proteome Science 8: 59.
19. Singhal, N., Sharma, P., Kumar, B., Sengupta, U., Venkatesan, K. and Bisht, D. (2010)
An in vitro infection model system to study proteins expressed during interaction of
mycobacterium with murine macrophages. Advances in Bioscience and Biotechnology 1:
180-184.
20. Singhal, N., Bisht, D. and Joshi, B. (2010) Immunoprophylaxis of tuberculosis: An
update of emerging trends. Arch. Immunol. Ther. Exp. 58: 97-106.
21. Singhal, N., Sharma, P., Kumar, M., Joshi, B. and Bisht, D. (2012) Analysis of
intracellular expressed proteins of Mycobacterium tuberculosis clinical isolates.
Proteome Science 10: 14.
22. Singh, I., Yadav, A.R., Mohanty, K. K., Katoch, K., Bisht, D., Sharma, P., Sharma, B.,
Gupta, U. D. and Sengupta, U. (2012) Molecular mimicry between Hsp65 of
Mycobacterium leprae and cytokeratin; Role in pathogenesis of leprosy. Cellular
Immunology 278: 63-75.
23. Kumar, B., Sharma, D., Sharma, P., katoch, V.M., Venkatesan, K. and Bisht, D. (2013)
Proteomic analysis of Mycobacterium tuberculosis isolates resistant to kanamycin and
amikacin. Journal of Proteomics 94: 68-77.
24. Parandhaman, D.K., Sharma, P., Bisht, D. and Narayanan, S. (2014) Proteome and
phosphoproteome analysis of the serine/threonine protein kinase E mutant of
Mycobacterium tuberculosis. Life Sciences 109: 73-128.
25. Lata, M., Sharma, D., Kumar, B., Deo, N., Tiwari, P. K., Bisht, D. and Venkatesan, K.
(2015) Proteome analysis of ofloxacin and moxifloxacin induced Mycobacterium
tuberculosis isolates by proteomic approach. Protein & Peptide Letters 22:362-71.
26. Singh, I., Yadav, A.R., Mohanty, K.K., Katoch, K., Sharma, P., Mishra, B., Bisht, D.,
Gupta, U.D. and Sengupta, U. (2015) Molecular mimicry between M.leprae proteins (50S
ribosomal protein L2 and lysyl-tRNA synthetase) and myelin basic protein: a possible
mechanism of nerve damage in leprosy. Microbes and Infection 17.
27. Singh, A., Gopinath, K., Sharma, P., Bisht, D., Sharma, P., Singh, N. and Singh, S.
(2015) Comparative proteomic analysis of sequential isolates of Mycobacterium
tuberculosis from a patient of pulmonary tuberculosis turning from drug sensitive to
multi-drug resistant. Indian Journal of Medical Research 141:27-45.
28. Gupta, R. K., Kumar, B., Bisht, D., Katoch, K., Mitra, K. and Srivastava, R. (2015)
Differentially expressed proteins in response to resuscitation of non-culturable cells of
Mycobacterium tuberculosis H37Rv: Potential new drug targets. International Journal of
Respiratory and Pulmonary Medicine 2; 3.
29. Sharma, D., Lata, M., Faheem, M., Khan, AU., Joshi, B., Venkatesan, K., Shukla, S. and
Bisht, D. (2015) Cloning, expression and correlation of Rv0148 to amikacin and
kanamycin resistance. Current Proteomics 12(2):96-100.
30. Lata, M., Sharma, D., Deo, N., Tiwari, P. K., Bisht, D. and Venkatesan, K. (2015)
Proteomic analysis of ofloxacin-mono resistant Mycobacterium tuberculosis isolates.
Journal of Proteomics, 127(Pt A):114-121.
31. Sharma, D., Kumar, B., Lata, M., Joshi, B., Venkatesan, K., Shukla, S. and Bisht, D.
(2015) Proteomic analysis of membrane fraction of kanamycin and amikacin resistant
Mycobacterium tuberculosis isolates. Plos One 10(10): e0139414.
32. Singhal, N., Kumar, M., Sharma, D. and Bisht, D. (2016) Protein profiling of
intraphagosomal expressed proteins of Mycobacterium bovis BCG. Protein & Peptide
Letters 23(1): 51-54.
33. Sharma, D and Bisht, D. (2016) An efficient and rapid method for enrichment of
lipophilic proteins from Mycobacterium tuberculosis H37Rv for two-dimensional gel
electrophoresis. Electrophoresis 37(9): 1187-1190.
34. Refaya, A.K., Sharma, D., Kumar, V., Bisht, D., Narayanan, S. (2016) A
Serine/threonine Kinase PknL, is involved in the adaptive response of Mycobacterium
tuberculosis. Microbiological Research (Accepted).
35. Qayyum, S., Sharma, D., Bisht, D. and Khan, A. U. (2016) Protein translation machinery
holds a key for transition of planktonic cells to biofilm state in Enterococcus faecalis: A
proteomic approach. Biochemical and Biophysical Research Communications
(Accepted).