Scientific Profile Format
Name of Scientist: Dr.H.B.Singh
Designation: Scientist B
Date of Birth: 15-01-1964
Discipline: Mycobacteriology
Address Office: Department of Microbiology and Molecular Biology,
National JALMA Institute for Leprosy and Other Mycobacterial Diseases (CMR),
TajGanj, Agra-282001
Address Residence: 6, Amarlok colony opp. JALMA. TajGanj, Agra-282004 Ph
0562-2232485, 9319071321,
Email Address: [email protected]
Date of Joining: 17 Sept., 1986
Date of Present Post: august. 2007
Department: Department of Microbiology and Molecular Biology
Educational qualification: M.Sc. Zoology (ent.) Ph.D NJIL & OMD Taj Ganj Agra
and Agra College Agra
Research Experience: 23 years
Area of Interest: Microbiology and Molecular Biology of Mycobacteria
Award/Honours, Fellowship:
Membership: IAL, IAMM, WHO fellowship awarded 2015
Seminars Conferences Attended: More than 30 national/ international
Publications: Total 33
1. Srivastava, K., Chauhan, D.S., Gupta, P., Singh, H.B., Sharma, V.D., Yadav, V.S.,
Sreekumaran Thakral, S.S., Dharamdheeran, J.S., Nigam, P., Prasad, H.K. and
Katoch, V.M. Isolation of Mycobacterium bovis and M.tuberculosis from cattle of
some farms in north India-possible relevance in human health. Indian J Med Res
128 (2008) 26.
2. Katoch, K., Katoch, V.M., Natrajan, M., Gupta, U.D., Sharma, V.D. and Singh, H
B. Long term follow-up results of 1 year MDT in MB leprosy patients treated with
standard MDT and once a month Minocycline and Ofloxacin. Indian J Lepr 80
(2008) 331.
3. Katoch, K., Singh, P., Adhikari, T., Benara, S.K., Singh, H.B., Chauhan, D.S.,
Lavania, M., Sachan, A.S. and Katoch, V.M. Potential of Mw as a prophylactic
vaccine against pulmonary tuberculosis. Vaccine. 26 (2008) 1228.
4. Chauhan, D.S., Sharma, V.D., Parashar, D., Chauhan, A., Singh, D., Singh, H.B.,
Das, R., Aggarwal, B.M., Malhotra, B., Jain, A., Sharma, M., Kataria, V.K.,
Aggarwal, J.K., Hanif, M., Shahani, A. and Katoch, V.M. Molecular typing of
Mycobacterium tuberculosis isolates from different parts of India based on IS6110
element polymorphism using RFLP analysis. Indian J Med Res 125 (2007) 577.
5. Das, R., Katoch, K., Jadaun, H.P.S., Gupta, P., Sharma, V.D., Malhotra, B. Garg,
A., Ayagiri, A., Chauhan, D.S., Singh, H.B., Yadav, P. and Katoch, V.M. Studies on
mutation in embB locus in Indian clinical isolates of Mycobacterium tuberculosis
having high degree of ethambutol resistance. Curr Sci 7 (2006) 923.
6. Das R, Gupta P, Singh H B, Sharma VD, Chauhan D S, Katoch K & Katoch
VM. Association mutations in rpsL gene with high degree of streptomycin
resistance in clinical isolates of Mycobacterium tuberculosis in India. Ind J Med
Res (2009), 129: 108-110.
7. Singh H B, Singh Pushpendra, Jadaun G P S, Srivastava K, Sharma V D, Chauhan
D S, Sharma S K, Katoch V M, Simultaneous use of two PCR systems targeting
IS6110 and MPB64 for confirmation of diagnosis of tuberculous lymphadenitis. J
Commun Dis 2006;38:274- 9
8. Lavania, M., Katoch, K., Sachan, P., Dubey, A., Kapoor, S., Kashyap, M.,
Chauhan, D.S., Singh, H.B., Sharma, V.D., Jadav, R.S. and Katoch, V.M. Detection
of Mycobacterium leprae DNA from soil samples targeting ELEP sequences. J
Commun Dis 38 (2006) 269.
9. Hirawati Katoch K, Chauhan D S, Singh H B, Sharma V D, Singh M, Kashyap M,
Katoch V M, Detection of M. leprae by reverse transcription- PCR in biopsy
specimens from leprosy cases: a preliminary study. J Commun Dis 2006;38:280- 7
10.Lavania Mallika, Katoch K, Sachan Pawan, Dubey Anuraag, Kapoor Shashi, Kashyap
Meera, Chauhan D S, Singh H B, Sharma V D, Jadhav R S, Katoch V M, Detection of
Mycobacterium leprae DNA from soil samples by PCR targeting RLEP sequences. J
Commun Dis 2006;38:269- 73
11.Lavania M, Katoch V M, Singh H B, Das Ram, Sharma V D, Chauhan D S, Natrajan M,
Katoch K, Genetic polymorphism among Mycobacterium leprae strains from northern
India, by using TTC repeats. Indian J Lepr 2005;77:60- 5
12.Srivastava, K., Das, R., Jakhmola, P., Chauhan, D.S., Sharma, V.D., Singh, H.B.,
Sachan, A.S. and Katoch, V.M. Correlation of mutations detected by INNO-LiPA
with levels of rifampicin resistance in Mycobacterium tuberculosis. Indian J Med
Res 120 (2004) 100.
13.Singhal, T., Om Prakash, Singh, H.B., Sharma, V.D. and Katoch,
V.M.Demonstration of circulating anti-LAM and anti-PGL antibodies, 35kDa
Mycobacterium leprae antigen and gene encoding for 36kDa protein in plasma
from leprosy patients. J Immunol Immunopathol 6 (2004) 127.
14.Natarajan, M., Katoch, K., Katoch, V.M., Sharma, V.D., Singh, D., Das, R.,
Chauhan, D.S. and Singh. H.B. In-situ hybridization in the histodiagnosis of early
and clinically suspicious leprosy. Int J Lepr (2004).
15.Singh HB, Katoch VM, Natrajan M, Sharma VD, Chauhan DS, Lavania M,
Sharma P, Sharma M, Katoch K, Benara S, Singh P. Improved protocol for PCR
detection of Mycobacterium leprae in buffered formalin-fixed skin biopsies. Int J
Lepr Other Mycobact Dis. 2004;72:175- 8
16.Das, R., Srivastava, K., Gupta, P., Sharma, V.D., Singh D., Chauhan, D.S., Singh,
H.B. and Katoch, V.M. Comparison of E-test with MIC method of Lownstein-
Jensen medium for susceptibility testing of Mycobacterium tuberculosis. Curr Sci
85 (2003) 191.
17.Dandapat, P., Verma, Rishendra, Venkatesan, K., Sharma, V.D., Singh. H.B. and
Katoch, V.M. Identification of the bound lipids on the cell envelopes of
Mycobacterium bovis. Indian J Anim Sci 72 (2002) 946.
18.Malhotra, B., Pathak, S., Vyas, L., Katoch, V.M., Srivastava, K., Chauhan, D.S.,
Singh, D., Sharma, V.D., Das, R. and Singh, H.B. Drug susceptibility profiles of
Mycobacterium tuberculosis isolates at Jaipur. Indian J Med Microbiol 20 (2002)
19.Singh, H.B., Chauhan, D.S., Singh, D., Das, R., Srivastava, K., Yadav, V.S., Anil
Kumar, Katoch, V.M. and Sharma, V.D. Rapid discrimination of Indian Isolates of
M.tuberculosis by Random Amplified polymorphic DNA (RAPD) analysis- A
preliminary report. Indian J Med Microbiol 20 (2002) 69.
20.Gupta, U.D., Katoch, K., Sharma, R.K., Singh, H.B., Natarajan, M., Singh, D.,
Sharma, V.D., Chauhan, D.S., Dass, Ram, Srivastava, K. and Katoch, V.M.
Analysis of quantitative relationship between viability determination in leprosy by
MFP, ATP bioluminescence and Gene amplification assays. Int J Lepr (2001).
21.Katoch, K., Katoch, V.M., Natarajan, M., Sharma, V.D., Singh, H.B. and Gupta,
U.D. Chemotherapy trials in MB leprosy using conventional and newer drugs
ofloxacin and minocycline. Indian J Dermatol Venereol Leprol 66(10) (2000) 18.
22.Gupta UD, Katoch K, Singh HB, Natrajan M, Sharma VD, Katoch VM.
Assessment of viability by normal mouse foot-pad and bacillary ATP
bioluminescence assay in multibacillary cases treated with an MDT regimen using
conventional as well as newer drugs like minocycline and ofloxacin. Indian J
Lepr. 2000;72:437- 42
23.Verma, R., Sharma, V.D., Singh H.B., Katoch, V.M. Characterization of strains of
M.bovis by protein electrophoregrams and isoenzyme patterns. Indian J Anim Sci
70 (2000) 37.
24.Verma, R., Singh, H. B., V.D. Sharma, Katoch, V.M. Chracterization of M bovis
strains by protein electrophoreasis and isoenzyme patterns. Indian J Anim Sci 69
25.Gupta, U.D., Sharma, R.K., Katoch, K., Natarajan, M., Singh, D., Sharma, V.D.,
Singh, H.B. and Katoch, V.M. Comparision of assessment of viability by normal
mouse foot pad and PCR. Indian J Med Microbiol 17(4) (1999) 187.
26.Dandapat P, Verma R, Venkatesan K, Sharma VD, Singh HB, Das R, Katoch VM.
Rapid detection of Mycobacterium bovis on its lipid profile by thin layer
chromatography. Vet Microbiol. 1999;65:145- 51
27.Katoch, V.M., Singh, D., Chauhan, D.S., Sharma, V.D. Singh, H.B., Das, R.,
Srivastava, K., New DNA fingerprinting techniques for TB- relevance in control.
Proc. Indo-French Symposium on "Multiple drug resistance in emerging and
reemerging discases" New Delhi 9 (1999) 85.
28.Gupta, U.D., Katoch, K., Singh, H.B., Natrajan, M., Sharma, V.D. and Katoch,
V.M. Detection of viable organisms in cases treated with multidrug treatment. Acta
Leprol.11(3) (1999) 89.
29.Singh HB, Katoch K, Natrajan M, Sharma RK, Gupta UD, Sharma VD, Singh D,
Chauhan DS, Srivastava K, Katoch VM. Effect of treatment on PCR positivity in
multibacillary leprosy patients treated with conventional and newer drugs
ofloxacin and minocycline. Acta Leprol. 1999;11:179- 82
30.Singh H.B., Sharma,V.D.and V M Katoch.PCR techniquesin Leprosy . CJIL Q Bull
18 (1998) 1-4
31.Gokhlesh Kumar. HB Singh, Kiran Shrivastava and V M Katoch. Screening of
Induian buffaloes for Mycobacterium tuberculosis complex by amplification of IS
1081 gene J Vet pub Hlth 3 (2005) 7-11
32.Prakash. H.B .Singh,S Rai, A. Pandey, B.K.Girdharand V.M.Katoch. Detection of
Mycobaterium Leprae DNAfor 36 KDa protein in urine from Leprosy patient. A
preliminary report. Rev Inst. Med. Trop. S Paulo 46 (2004) 275-277
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