CURRICULAM VITAE
DR. VIRENDRA KUMAR SHARMA,
Scientist-D
M.Sc., M.Phil., Ph.D
MAILING ADDRESS
Scientist-D
Head, Department of Electron Microscopy
National JALMA Institute for Leprosy &
Other Mycobacterial disease
Designation:Presently working as Scientist-Din National JALMA Institute for Leprosy & Other
Mycobacterial Diseases, (Indian Council of Medical Research) Ministry of Health India.
Profile: Research:Workingwith infectious diseases & guiding Ph.D students, Project and
Training students who wish to do research work in medical fields.Attended National &
International Conferences, Seminars, Workshop and deliver lectures & invited talk in India and
many foreign countries. We got an opportunityto take my basictraining by eminent scientist
Prof. Mitsugu Nishiura, from Kyoto University, Japan for more than 2 years pertaining to
progression of various infectious diseases.The students who are doing M.Sc. (Biochemistry,
Biotechnology, and Microbiology students of various Universities were taught basic tool and
techniques and principals of electron Microscopy including various medical testing.Deputed as
an external examiner in many national & international Universities to take practical
examinations of graduate and undergraduate biomedical, Ph.D & project students. We are also
helping to the project &Ph.D. students by providing the laboratory facilities for completion of
their research work.Imparting the summer training to the students on various laboratory
techniques pertaining to the processing of specimens and cutting semi-thin and ultra-thin
sections, including the shadowing of the mycobacteria for characterization. Therefore, I have 30
years R&D experiences from national & international reputated Institutions.
Some Important handled Projects:
(i)Role of biofilm in Mycobacteria: Correlation of Ultrastructural and molecular
profile.D.B.T.funded project as Principal investigator project
No.BT/PR10435/Med/30/85/2008
(ii)Development and formulation of novel bioactive nanosilver phytochemical
combination with potential against multidrug resistance microbial pathogens and its
efficacy for disease control. D.B.T. funded Project as Co- Principal Investigator
project No.BT/PR10880/NNT/28/141/2008.
(iii)Mechanism of endothelial cell infection and their role in transmitting the diseases in
peripheral nerves in leprosy. (Intramural Project).
Professional Experience:
I have joined the National JALMA Institute for leprosy & Other Mycobacterial Diseases in 24
th
Feb.
1984. Japanese NGOs made this Institute in 1967, JALMA abbreviates as ”JAPANESE
ASSOCIATION OF LEPROSY MISSION FOR ASIA”. This Institute handed over to the Government
of India (Indian Council of Medical Research) Ministry of Health in 1976 and still is working under the
banner of Japanese Government as “National JALMA Institute for leprosy & Other Mycobacterial
Disease”. The Institute is participating in and coordinating several multicentric studies in the field of
Mycobacterial Disease, Tuberculosis, Leprosy,&also a reference center for HIV infection. Currently the
Institute has launched major initiatives using modern tools of genomic for investigating strain diversity,
mechanisms of drug resistance and host parasite interaction in leprosy, tuberculosis and other
mycobacterial diseases at molecular and ultrastructural level.
1984--1985 I got an opportunity to take my initial training for more than a year under Prof. M.
Nishiura, an eminent Electron microscopist, Kyoto University, JAPAN pertaining to Processing
various specimens for SEM/TEM, cutting semi/cryo/ultrathin sections, freez-fracture of various types of
specimens/biopsies. I have learned maintenance and operation of Electron microscope including film
processing, printing and interpretation of electron micrographs.
1985-1986 I had the privileged to take the training on “Cell Ultra structural and Electron Microscopy”
under Prof. B.K.Ghosh, Ruttgers University, New Jersey, U.S.A. in WHO workshop at NICED,
Kolkata India.
1986-1990 I worked with Dr. R. B. Narayanan, (presently he is in the Director, Department of
Immunology, Anna University, Chennai) in the area pertaining to the Immunopathological Techniques
and Characterisation of Cells in Granuloma Formation during the disease progress. We have published
some research papers in highly impacted foreign journals (List enclosed).
2000 I worked on “Micro structural characterization” using EDS techniques in the Scanning Electron
Microscope at Environmental Technology Institute, SINGAPORE.
Presentedresearch papers in various International conference/Seminar/ workshop/meetingSalzburg/
AUSTRIA, JAPAN, THAILAND, SINGAPORE.
Presently I am working as Scientist-D/Asstt.Directorand looking after the Electron Microscope
Department since last 30 years and published some interesting research papers in highly impacted
foreign journals (List enclosed).
Some Important Publications on infectious diseases
V. Kumar and D.P.S. Bhati. Histochemical observations of alkaline phosphatase in the pituitary of
HemidactylusflaviviridisRuppel. Bulletin Pure and Applied Sciences 24P: 42-45, (1983)
V. Kumar, G.N. Malaviya , R.B. Narayanan, and M. Nishiura. Ultrastructural Studies of peripheral
nerves in lepromatous leprosy patients. Ind. J. Lepr. 60: 360-363 (1988).
V.Kumar, R.B. Narayanan and G.N. Malaviya. Ultrastructural characteristics of Macrophages in
dermal leprosy granulomas. Japanese J.Lepr.58:(4)1985-190 (1989).
V.Kumar, R.B.Narayanan, B.K.Girdhar and G.N.Malaviya. An ultrastructural study of blood vessels in
peripheral nerves of leprosy patients. Japanese. J.Lepr. 58: (3) 179-185 (1989).
V.Kumar, R.B.Narayanan, B.K.Girdhar and G.N.Malaviya. Isolation and characterization of infiltrates
in the nerves of Neuritic leprosy. ActaLeprologica. 7 : 92) 157-161 (1990).
V.Kumar, R.B.Narayanan and G.N.Malaviya. An ultrastructural study of Schwann cells in peripheral
nerves of leprosy patients. Ind. J. Lepr. 64 : 81-87 (1992).
V.Kumar, K. Katoch, V.M.Katoch, V.P.Bharadwaj. A preliminary study of correlation of immuno-
histological and ultrastructural characteristics of neural granuloma in leprosy patients. Acta. Leprologica,
8: (2), 87-94 (1992).
Parkash.O, Kumar V. Membrane attack complex in thickened cutaneous sensory nerves of leprosy
patients. Acta. Leprologica, 9: (4) 195-199 (1995).
V.Kumar. Ultrastructural demonstration of clofazimine crystals in Schwann cells from lepromatous
leprosy. Microscopy & Analysis 68 : 29-31 (1998).
V.Kumar. Ultrastructural studies of granuloma in leprosy : JALMA Experience. JALMA Quarterly
Bullatin21 : 3 (1999)
V.Kumar, and U.Sengupta. Ultrastructural study of Schwann Cells and Endothelial cells in
pathogenesis of leprous neuropathy. Int. J. Lepr. 71(4) 328-340 (2003).
V.Kumar, High resolution shadowing of Mycobacterium leprae. Biotechnic&Histochemistry. 79 5/6:
197-201 (2004).
V.Kumar, V.K.SharmaAn ultrastructural study of the interaction ofM.leprae with host cell.
Microscopy & Analysis. 54:5-7 (2006)
Kumar. V, Does clofazamine (B663) reach Mycobacteriumleprae persisting in Schwann cells and
endothelial cells of endoneurial blood vessels in peripheral nerves ? 71:Microscope Research &
techniques 71:614-618 (2008)
Kumar,VHigh resolution structural characterizations of Mycobacterium leprae by Shadow-casting
techniques. EMSI Bulletin 7: 7-9 (2008).
Islam. B., Khan S.N., Naeem. A, Kumar.V, A.U.Khan. Novel effect of plant lectins on the
inhibition of Streptococcus mutans biofilm formation on saliva-coated surface. J. Appli.Micro.
ISSN: 1364-1372(2009).
Jain A, Bhadauria S., Kumar V, Chauhan RS. Biodeterioration of sandstone under the
influence of different humidity levels in laboratory conditions. Build.& Environ. 44:
1276-1284.
Kumar V.,SachanT., Sharma Ankit, Dixit S. &Upadhyay M. (2011) Ultrastructural
studies of the Clofazamine deposited in Schwann cells and Endothelial cells in peripheral
nerves of treated leprosy patients. Ind.Res.J. genet.&bioech. 1(3):101-109 (2009).
Manish Dubey, Seema BhadauriaKumar V & V. M. Katoch. Antibacterial Activity of Biologically
Synthesized Nanosilver against Drug-Resistant Bacterial Pathogens. Int.J.Gr. Nanotec. 4:2,174-182,(
2012).
Ankit Sharma &Kumar V. Improved production of Secondary metabolites by fermentation.
Ind.Res.J.genet& biotech.4:(4), 242- 249(2012).
Kumar V., T. Sachan, M. Natarajan and Ankit Sharma. High resolution structural changes of Schwann
cells and endothelial cells in peripheral nerves across leprosy spectrum. Ultra. Pathol. 1-7, (2014).
Kumar V., T. Sachan, P.Sharma and K.D.Rawat. Ultrastructural morphologic changes in mycobacterial
Biofilm in different extreme conditions. Ultra.Pathol.1-11(2014).
T.Sachan and Kumar V.Antibiotic susceptibility in Biofilms ofM.SmegmatisInt.J.Appl.Sci.Biotechnol.
3(34):635-641 (2015).
T.Sachan and Kumar V. et al. Chemical and Ultrastructural Characteristics of Mycobacterial Biofilms.
Asian.J Ani.& Vet. Adv. 10(10):592-622. (2015)
Conferences / Workshops / SeminarsAttended
Attended WHO workshop on “Cell Ultrastructure and Electron Microscopy” at the National Institute of
Cholera and Enteric Disease (ICMR) Calcutta organized by Prof.B.K.Ghose, RuttgersUniversity,USA on
6
th
August, 1985.
Participated in the group discussion on “Early recognition of nerve damage in leprosy” in Indo-UK
workshop on leprosy held at JALMA, Agra in Feb.1989.
Attended Indo-UK Symposium on leprosy held at the Central JALMA Institute for leprosy (ICMR) on
April 7-10, 1986.
Presented the work on nerve granuloma in Indo-US workshop on CMI in relation to tropical disease
held at CDRI, Lucknow in Oct.1988.
Attended 15
th
Biennial IAL Conference and presented a paper on “Ultrastructural studies of peripheral
nerves in lepromatous leprosy patients” at Vishakhapatnam in Dec,1987.
Attended the 15th annual conference of the Indian Immunological SocietyinSiliguri and presented a
paper on “Comparison of the characteristic of cells n the skin and nerve granuloma of leprosy patients”
held at 28
th
Nov.,1988.
Participated in the 16
th
National Congress of Indian Association of Medical microbiologist and
presented a paper entitled “An Ultrastructural study of blood vessels of leprosy in peripheral nerves”
held at Aurangabad from 23-25
th
Oct., 1991.Attended Indo-UK workshop on leprosy research held at
the Central JALMA Institute for leprosy (ICMR) Feb. 15-17
,
1989.
Attended 14
th
National congress of Indian Association of Medical Microbiologists And presented a
paper on “A study on the Ultrastructure morphology of M. leprae” held at CMC Vellore on 27
th
Oct.,1990.
Participated in Continuing Medical Education (CME) programfor use of computer for Microbial data
handling held at Aurangabad on 22
nd
Oct.,1991.
Participated in the Biennial Conference of Indian Association of leprologists and presented a paper
entitled “Ultrastructural study of Macrophage in the dermal leprosy granuloma of leprosy patients” held
inDurg, Bhilai on 2-5 Jan, 1992.
Attended workshop on advances in Laboratory Techniques with special care of leprosy patients held at
5
th
Jan., 1992 at DurgBhilai.
Participated in the CME program on “Leishmaniasis and leprosy” held in Calcutta on 16-17
th
Dec.,
1993.
Participated in the 17
th
National congress of IAMM and presented a paper entitled “A preliminary study
of correlation Immunohistological and Ultrastructural characteristics of neural granuloma in leprosy”
held at Calcutta on 18-20
th
Dec., 1993.
Attended the CME on Vaccines state of art and prospective in India held at Pune on 11
th
Nov. 1994.
Participated in the 18
th
National Congress of Indian Association of Medical Microbiology held at Pune
and presented a paper entitled “Ultrastructural Characteristics of peripheral nerves in leprosy patients”
on 12-14
th
Nov.,1994.
Attended the XIV Annual Conference of the Indian Academy of neurosciences held at Bombay from 8-
2-96 to 10-2-96 and presented a paper entitled “Ultrastructural demonstration of Clofazimine crystals in
Schwann cells from lepromatous leprosy patients”.
Participated in the XX National Congress of IAMM in Agra from 31-10-1996 and also attended the
workshop and presented a paper entitled “Macrophages in the dermal leprosy granuloma: An electron
microscopic study” and also (acted as reporters) at Central Jalma Institute Agra.
Attended pre-workshop on “Micro structural characterization” using EDS techniques in the Scanning
Electron Microscope at Environmental Technology Institute, Singapore on 25
th
June, 2000.
Participated in VIIth Asia Pacific electron microscopy conference held at Singapore from 26-6-2000 to
30-6-2000 and presented a paper entitled “Electron microscopic study of Clofazimine crystals in
Schwann cells from leprosy patients”.
Participated in XXV annual conference of the Electron microscope Society of India on Electron
microscopy and allied fields and presented a paper entitled “Efficacy of the drugs on the Morphology of
M. leprae: an Electron microscopic study” on 20
th
to 22
nd
February, 2002 at I IT- Bombay.
Participated in Ranbaxy Science Foundation, 10
th
Round Table Conference on “ Leprosy
Elimination-Critical issues” held at Agra on September 28, 2002.
Participated in XXVI Annual conference on Electron microscopy and allied fields of Electron
microscope Society of India and presented a paper entitled “Ultrastructural studies of cellular and
vascular changes in leprous neuritis” on April 16-18, 2003 at Central Potato Research Institute, Shimla-
171001 (H.P.)
Participated in 30
th
Annual meeting of Society for Cutaneous Ultrastructure Research (SCUR) and
presented a research paper entitled “Mechanism of endothelial cell infection and their role in
transmitting the disease in peripheral nerves in leprosy patients” on 24
th
to 26 April 2003 in Salzburg /
AUSTRIA.
Attended the Indian Association of leprosy national workshop on the diagnosis and management of
leprosy in the context of integration of leprosy program with general health services Agra from 18
th
to
19
th
October 2003 at JALMA Agra.
Participated the 23
rd
biennial conference of Indian association of leprologist and presented a paper
“Ultrastructural study of Schwann cell and Endothelial cell in leprosy patients” at Haldia Port city, West
Bengal, On 27
th
to 29
th
February 2004.
Attended the meeting on Resources Material on IPR and WHO issue organized by Indian Council of
Medical Research at Central JALMA Institute for Leprosy and Other Mycobacterial Disease in March
2003/
Attended the 27
th
Annual Meeting of Electron Microscope Society of India and conference on electron
Microscopy and allied fields and presented a paper entitled “Ultrastructural study of Host cells in
Leprosy infection at National Physical Laboratory New Delhi on 1
st
April to 3
rd
April 2004.
Attended Indian Association of Leprosy and presented a paper entitled “Electron microscopic study of
peripheral nerves in treating lepromatous leprosy patients” in November 19-21 2007 at Kanpur
university
Presented a research paper in the Conference on Electron microscopy &XXIXth Annual meeting of
EMSI in November 26-28, 2007 at Delhi university.
Participated in the training of Scanning Electron microscope from 5
th
February to 9
th
February 2007 at
Hitachi High-Technologies Corporation Tokyo, Mito, Japan.
Participated in the training of Scanning Electron microscope from 12
th
February to 16
th
February 2007
at Hitachi High-Technologies Singapore.
Participated in the training of Transmission Electron microscope from 19
th
February to 23
rd
February
2007 at Coax Group Corporation Bankok, Thailand.
Participated in workshop on Electron microscope for diagnostic & biomedical research
anddeliverya invited talk at the National Institute of Pathology New Delhi on dated September
23-27, 2013.
Participated CME workshop at theJamiyaHamdardUniversity, New Delhi and deliveran invited
talkon “Applications of Electron microscope in Infectious diseases” 21th December 2013.
Participated in International Conference on Electron microscopy &xxxv Annual meeting of
Electron microscope Society of India, deliveran invited talk and chair the session of infectious
diseases held at the University of Delhi on July 9-11, 2014.
Participated in International Conference on Nanostructured Materials & Nanocomposites and
deliveran invited talk and chair the session infectious diseases held at Hindustan College of
Science & Technology, 12-14 December, 2015.
Participated in International Conference on Emerging trends in Biotechnology & Science with
especial reference to climatic changes organized by Indian Society of Genetics, Biotechnology
Research & Development at Banasthali University Rajasthan, February18-20, 2015.
Participated in 2
nd
International Conference on New challenges in Biotechnology & Molecular
biology in the context of 21
st
century organized by Indian Society of Genetics, Biotechnology
Research & Development and deliveran invited talk and chair the session held at St.Johns
College Agra February 27-29, 2016.